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Journal article
Age-related remodeling of the vocal fold extracellular matrix composition, structure, and biomechanics during tissue maturation
Connective tissue research, pp 1-14
12 Dec 2024
PMID: 39665313
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The vocal folds (VFs) are among the most mechanically active connective tissues, vibrating between 80 and 250 hz during speech. Overall VF function is determined by the composition and structure of their extracellular matrix (ECM). During tissue maturation, the VFs remodel from a monolayer of collagen fibers to a tri-layered structure, affecting tissue biomechanics. However, age-related VF ECM remodeling remains poorly understood since few studies have explored the proteins governing collagen fibrillogenesis or the non-collagenous ECM components critical for VF elasticity.
VFs from immature, sexually mature, and skeletally mature rats were evaluated by endoscopy, histology, and electron microscopy for cellular and biochemical composition, ECM organization, and proteoglycan distribution. Nanoindentation modulus was determined by atomic force microscopy.
Collagen fiber abundance, maturity, and alignment are low in immature rats but show an age-dependent increase during tissue maturation. Lumican and fibromodulin, which regulate early-stage collagen fibril formation, are distributed throughout the VFs, and their abundance decreases with age. Decorin, involved in collagen organization, is concentrated just beneath the epithelium and increases with age. Elastin levels increase during tissue maturation, but hyaluronic acid abundance and distribution remain consistent with age. VF nanoindentation modulus trends toward a decrease with age.
This work identifies changes in VF ECM composition and organization during tissue maturation, focusing on proteins that regulate collagen fibrillogenesis, fiber assembly, and VF biomechanics. These findings may inform the development of pro-reparative therapies designed to influence collagen network structure and overall ECM dysregulation in a number of laryngeal pathologies.
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Details
- Title
- Age-related remodeling of the vocal fold extracellular matrix composition, structure, and biomechanics during tissue maturation
- Creators
- Ryan M Friedman - Children's Hospital of PhiladelphiaArielle S Breuninger - Children's Hospital of PhiladelphiaMatthew R Aronson - Children's Hospital of PhiladelphiaElizabeth A Brown - Children's Hospital of PhiladelphiaNeil Patel - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA, USALin Han - Drexel UniversityKaren B Zur - University of PennsylvaniaRiccardo Gottardi - Ri.MED
- Publication Details
- Connective tissue research, pp 1-14
- Publisher
- Taylor & Francis
- Number of pages
- 14
- Grant note
- Children's Hospital of Philadelphia Research InstituteFrontier Program in Airway Disorders of the Children's Hospital of PhiladelphiaRi.MED FoundationCenter for Undergraduate Research and Fellowships at the University of PennsylvaniaDepartment of Bioengineering of the University of Pennsylvania (Noordergraaf Fellowship)National Science Foundation (NSF): CMMI-1751898 National Science Foundation Graduate Research Fellowship: DGE 1845298 U. S. Department of Education's Graduate Assistance in Areas of National Need (GAANN) Program Fellowship
This work was supported in part by the Children's Hospital of Philadelphia Research Institute (RG and KBZ), the Frontier Program in Airway Disorders of the Children's Hospital of Philadelphia (RG), the Ri.MED Foundation (RG), the Center for Undergraduate Research and Fellowships at the University of Pennsylvania (ASB), the Department of Bioengineering of the University of Pennsylvania (Noordergraaf Fellowship to EAB), the National Science Foundation (NSF) CMMI-1751898 (LH), the National Science Foundation Graduate Research Fellowship No. DGE 1845298 (RMF and MRA), as well as Drexel University's Research and Engineering for Pediatrics by Interdisciplinary Collaboration Leveraging Education and Partnerships for Pediatric Healthcare (R-EPIC LEAP for Pediatric Healthcare) from the U. S. Department of Education's Graduate Assistance in Areas of National Need (GAANN) Program Fellowship (NP). The authors have no other funding, financial relationships, or conflicts of interest to disclose.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001375887600001
- Scopus ID
- 2-s2.0-85211634814
- Other Identifier
- 991022005187104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology
- Orthopedics