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Aged Micro-environment Severely Impairs Specific CD8 T Cell Response to Virus Infection (85.21)
Journal article   Peer reviewed

Aged Micro-environment Severely Impairs Specific CD8 T Cell Response to Virus Infection (85.21)

Jiu Jiang, Andrew J. Bennett and Donna M. Murasko
The Journal of immunology (1950), v 178(1_Supplement), pp S122-S122
01 Apr 2007
DOI: https://doi.org/10.4049/jimmunol.178.Supp.85.21

Abstract

Abstract Both intrinsinc and extrinsinc factors are considered to affect immunity with aging. However, few studies have reported that the aged micro-environment affects the specific CD8 T cell response to primary virus infection. In this study, we adoptively transferred CFSE-labeled young P14 cells (2-month-old) into young (4-month-old) and aged (24-month-old) mice. The recipients were infected with LCMV (Arm strain) and the Db-GP33–41 specific CD8 T cell response in the spleen was examined for virus specific responses using flow cytometric analysis of Db-GP33–41 tetramer binding and intracellular IFN-γ production at different times after infection. No obvious expansion of the P14 cells was observed on days 1 and 2 after infection in either age group. However, on day 3 post-infection, extensive expansion of P14 cells was observed in young, but not aged, recipients (increased percentage: young vs aged: 275% vs 18%). Coincidentally, 68% percent of CD8 T cells of young recipients were depleted, while no CD8 T cells of aged recipients were depleted on day 3. These results confirm our previous data with E55+MuLV infection, that non-specific depletion of CD8 T cells occurs early after infection in young but not aged mice, and support the hypothesis that this lack of depletion contributes to the hyporesponsiveness of aged mice. While our previous data demonstrated that the aged environment limits the depletion of T cells of young mice, the current data expands these findings by suggesting that the aged environment also limits expansion of virus-specific T cells of young mice. This work was supported by National Institute of Health Grant AG14913 to D. M. M.

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