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Journal article
Aleuria Aurantia Lectin (AAL)-Reactive Immunoglobulin G Rapidly Appears in Sera of Animals following Antigen Exposure
PloS one, v 7(9), pp e44422-e44422
14 Sep 2012
PMID: 23024749
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have discovered an Aleuria Aurantia Lectin (AAL)-reactive immunoglobulin G (IgG) that naturally occurs in the circulation of rabbits and mice, following immune responses induced by various foreign antigens. AAL can specifically bind to fucose moieties on glycoproteins. However, most serum IgGs are poorly bound by AAL unless they are denatured or treated with glycosidase. In this study, using an immunogen-independent AAL-antibody microarray assay that we developed, we detected AAL-reactive IgG in the sera of all animals that had been immunized 1-2 weeks previously with various immunogens with and without adjuvants and developed immunogen-specific responses. All of these animals subsequently developed immunogen-specific immune responses. The kinetics of the production of AAL-reactive IgG in mice and rabbits were distinct from those of the immunogen-specific IgGs elicited in the same animals: they rose and fell within one to two weeks, and peaked between four to seven days after exposure, while immunogen-specific IgGs continued to rise during the same period. Mass spectrometric profiling of the Fc glycoforms of purified AAL-reactive IgGs indicates that these are mainly comprised of IgGs with core-fucosylated and either mono- or non-galactosylated Fc N-glycan structures. Our results suggest that AAL-reactive IgG could be a previously unrecognized IgG subset that is selectively produced at the onset of a humoral response.
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Details
- Title
- Aleuria Aurantia Lectin (AAL)-Reactive Immunoglobulin G Rapidly Appears in Sera of Animals following Antigen Exposure
- Creators
- Songming Chen - Hepatitis B FoundationChen Lu - Hepatitis B FoundationHongbo Gu - Brown UniversityAnand Mehta - Drexel UniversityJianwei Li - Thomas Jefferson UniversityPatrick B. Romano - Drexel UniversityDavid Horn - Hepatitis B FoundationD. Craig Hooper - Thomas Jefferson UniversityCarthene R. Bazemore-Walker - Brown UniversityTimothy Block - Drexel University
- Publication Details
- PloS one, v 7(9), pp e44422-e44422
- Publisher
- Public Library Science
- Number of pages
- 10
- Grant note
- U01CA168856 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) HDTRA1-07-RDINO-BAA / Department of Defense; United States Department of Defense
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000308860100007
- Scopus ID
- 2-s2.0-84866385852
- Other Identifier
- 991019167594304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology