Journal article
All-liquid separations, protein microarrays, and mass spectrometry to interrogate serum proteomes: an application to serum glycoproteomics
Methods in molecular biology (Clifton, N.J.), v 520, pp 75-87
2009
PMID: 19381948
Abstract
Disease-related changes in serum proteins are reasonable targets for early detection particularly due to the noninvasive approach in obtaining samples. Glycoproteins specifically have been implicated in a variety of disease types ranging from immune diseases to cancers. High-throughput screening methods that can assess glycosylation states of all serum proteins in normal and diseased sample groups can facilitate early detection as well as shed light on disease progression mechanisms. Outlined here is a combination of liquid separation, protein microarray, and mass spectrometry approach to highlight candidate proteins involved in diseases through glycosylation mechanisms.
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5 citations in Scopus
Details
- Title
- All-liquid separations, protein microarrays, and mass spectrometry to interrogate serum proteomes: an application to serum glycoproteomics
- Creators
- Tasneem H Patwa - University of MichiganYinghua Qiu - University of MichiganJia Zhao - University of MichiganDiane M Simeone - University of MichiganDavid M Lubman - University of Michigan
- Publication Details
- Methods in molecular biology (Clifton, N.J.), v 520, pp 75-87
- Publisher
- Springer Nature
- Grant note
- R01CA106402 / NCI NIH HHS R016M49500 / PHS HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Scopus ID
- 2-s2.0-65649106894
- Other Identifier
- 9781603278119; 1603278117; 991021448050904721