Journal article
Alpha interferon-induced antiviral response noncytolytically reduces replication defective adenovirus DNA in MDBK cells
Antiviral research, v 76(3), pp 232-240
Dec 2007
PMID: 17897730
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Although alpha interferon (IFN-alpha) is of benefit in the treatment of viral hepatitis B, HBV replication has been refractory to the cytokine in commonly used hepatocyte-derived cell lines. In search for a cell culture system to study the mechanism by which IFN-alpha inhibits HBV replication, we infected a variety of cell lines with an adenoviral vector containing a replication competent 1.3-fold genome length HBV DNA (AdHBV) and followed by incubation with IFN-alpha. We found that IFN-alpha efficiently decreased the level of HBV DNA replicative intermediates in AdHBV infected Madin-Darby bovine kidney (MDBK) cells. Further analysis revealed, surprisingly, that IFN-alpha did not directly inhibit HBV replication, rather the amount of adenovirus DNA in the nuclei of MDBK cells was reduced. As a consequence, HBV RNA transcription and DNA replication were inhibited. Experiments with adenoviral vector expressing a green fluorescent protein (GFP) further supported the notion that IFN-alpha treatment noncytolytically eliminated adenovirus DNA, but did not kill the vector infected MDBK cells. Our data suggest that IFN-alpha-induced antiviral program is able to discriminate host cellular DNA from episomal viral DNA and might represent a novel pathway of interferon mediate innate defense against DNA virus infections.
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Details
- Title
- Alpha interferon-induced antiviral response noncytolytically reduces replication defective adenovirus DNA in MDBK cells
- Creators
- Ju-Tao Guo - Drexel UniversityTianlun Zhou - Hepatitis B FoundationHaitao Guo - Drexel UniversityTimothy M Block - Drexel University
- Publication Details
- Antiviral research, v 76(3), pp 232-240
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000251100200003
- Scopus ID
- 2-s2.0-35348969739
- Other Identifier
- 991019168153604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Pharmacology & Pharmacy
- Virology