Journal article
Alteration in Host Cell Tropism Limits the Efficacy of Immunization with a Surface Protein of Malaria Merozoites
Infection and immunity, v 73(10), pp 6363-6371
Oct 2005
PMID: 16177307
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Immunization with
Plasmodium yoelii
merozoite surface protein-8 (PyMSP-8) has been shown to protect mice against lethal
P. yoelii
17XL malaria. Here we demonstrate that PyMSP-8-specific antibodies preferentially suppress
P. yoelii
17XL growth in mature erythrocytes compared to growth in reticulocytes and do not suppress the growth of nonlethal
P. yoelii
17X, a parasite that primarily replicates in reticulocytes. The protection against normocyte-associated
P. yoelii
malaria parasites is mediated by antibodies that recognize conformational epitopes of PyMSP-8 that are nonpolymorphic. We examined changes in gene expression in reticulocyte-restricted
P. yoelii
17XL parasites that escaped neutralization by PyMSP-8-specific antibodies using
P. yoelii
DNA microarrays. Of interest,
Pymsp-8
gene expression decreased, while the expression of
msp-1
,
msp-7
, and several rhoptry protein genes increased. Breakthrough parasites also exhibited increases in the expression of a subset of
yir
and
Pyst-a
genes that are predicted to encode polymorphic antigens expressed on the surface of infected erythrocytes. These data suggest that changes in the expression of parasite proteins expressed on the merozoite surface, as well as the surface of infected erythrocytes, may alter host cell tropism and contribute to the ability of malaria parasites to evade merozoite-specific, neutralizing antibodies.
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Details
- Title
- Alteration in Host Cell Tropism Limits the Efficacy of Immunization with a Surface Protein of Malaria Merozoites
- Creators
- Qifang Shi - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129Amy Cernetich - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129Thomas M Daly - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129Gina Galvan - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129Akhil B Vaidya - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129Lawrence W Bergman - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129James M Burns - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129
- Publication Details
- Infection and immunity, v 73(10), pp 6363-6371
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; [Retired Faculty]
- Web of Science ID
- WOS:000232087600017
- Scopus ID
- 2-s2.0-25444508440
- Other Identifier
- 991014878489904721
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- Web of Science research areas
- Immunology
- Infectious Diseases