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Alterations in hippocampal voltage-gated calcium channel α 1 subunit expression patterns after kainate-induced status epilepticus in aging rats
Journal article   Peer reviewed

Alterations in hippocampal voltage-gated calcium channel α 1 subunit expression patterns after kainate-induced status epilepticus in aging rats

Kevin M. Kelly, Milos D. Ikonomovic, Eric E. Abrahamson, Elena A. Kharlamov, Teresa M. Hentosz and David M. Armstrong
Epilepsy research, v 57(1), pp 15-32
2003
DOI: https://doi.org/10.1016/j.eplepsyres.2003.10.007
PMID: 14706730
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Aging Calcium channels Hippocampus Immunohistochemistry Kainate Status epilepticus
Young adult and aged male Fisher 344 rats underwent kainate-induced convulsive status epilepticus (SE) for 4 h prior to sacrifice to determine potential aging-related differences in the effect of prolonged SE on the expression of hippocampal voltage-gated calcium channels (VGCCs). Immunohistochemistry was performed on hippocampal sections using antibodies directed against the α 1 subunit of class A–D VGCCs. Compared to age-matched controls, SE animals showed a marked loss of α 1A immunoreactivity (IR) in CA3 and the hilus, which was more prominent in aged animals. α 1B-IR was decreased selectively in the stratum lucidum of CA3. α 1C-IR was increased on neuronal somata in the pyramidal and granule cell layers of both age groups. In contrast, there was a marked decrease of α 1C-IR in the neuropil of CA3 stratum pyramidale and portions of CA1, which was more pronounced in aged animals. α 1D-IR was decreased in CA3 and the hilus, which was more prominent in aged animals. Nissl staining demonstrated mild somal dysmorphia in the pyramidal cell layer of CA3, which was more apparent in aged animals. Fluoro-Jade B staining was prominent in the stratum pyramidale of CA3 and in the hilus of aged SE animals. These results demonstrated that expression patterns of hippocampal high-threshold VGCC α 1 subunits were altered variably during prolonged convulsive SE and were associated with prominent early degenerative changes in aged neurons in CA3 and the hilus.

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Domestic collaboration
Web of Science research areas
Clinical Neurology
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