Journal article
Altered Hemodynamics in the Embryonic Heart Affects Outflow Valve Development
Journal of cardiovascular development and disease, v 2(2), pp 108-124
01 Jun 2015
PMID: 26878022
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cardiac valve structure and function are primarily determined during early development. Consequently, abnormally-formed heart valves are the most common type of congenital heart defects. Several adult valve diseases can be backtracked to abnormal valve development, making it imperative to completely understand the process and regulation of heart valve development. Epithelial-to-mesenchymal transition (EMT) plays an important role in the development of heart valves. Though hemodynamics is vital to valve development, its role in regulating EMT is still unknown. In this study, intracardiac hemodynamics were altered by constricting the outflow tract (OFT)/ventricle junction (OVJ) of HH16-17 (Hamilton and Hamburger (HH) Stage 16-17) chicken embryos, ex ovo for 24 h. The constriction created an increase in peak and time-averaged centerline velocity along the OFT without changes to volumetric flow or heart rate. Computational fluid dynamics was used to estimate the level of increased spatially-averaged wall shear stresses on the OFT cushion from AMIRA reconstructions. OFT constriction led to a significant decrease in OFT cushion volume and the number of invaded mesenchyme in the OFT cushion. qPCR analysis revealed altered mRNA expression of a representative panel of genes, vital to valve development, in the OFT cushions from banded hearts. This study indicates the importance of hemodynamics in valve development.
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Details
- Title
- Altered Hemodynamics in the Embryonic Heart Affects Outflow Valve Development
- Creators
- Vinal Menon - University of South CarolinaJohn F. Eberth - University of South CarolinaRichard L. Goodwin - University of South CarolinaJay D. Potts - University of South Carolina
- Publication Details
- Journal of cardiovascular development and disease, v 2(2), pp 108-124
- Publisher
- Mdpi
- Number of pages
- 17
- Grant note
- University of South Carolina Research Development Fund grant P30GM103342 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) P20RR016434 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) FirstString Research Inc. P20GM103499 / NIH IDeA Networks of Biomedical Research Excellence (INBRE) grant for South Carolina
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000457902100006
- Scopus ID
- 2-s2.0-84971560223
- Other Identifier
- 991021902596504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cardiac & Cardiovascular Systems