Journal article
An Altered Maturation and Adhesion Phenotype of Dendritic Cells in Diseased Individuals Compared to Asymptomatic Carriers of Human T Cell Leukemia Virus Type 1
AIDS research and human retroviruses, v 29(9), pp 1273-1285
Sep 2013
PMID: 23750452
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The immunopathogenic mechanisms underlying human T cell leukemia virus type 1 (HTLV-1)-mediated diseases such as adult T cell leukemia (ATL) and HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) are not clearly understood. As critical effectors of antiviral immune response, dendritic cells (DCs) are implicated to play an important role in determining the outcome of HTLV-1 infection. However, a complete understanding of their role in any disease pathogenesis requires extensive assessment of the phenotypic and functional state of DCs. To enable this, we developed a polychromatic antibody cocktail comprising key phenotypic and functional markers of DCs and applied it in a patient cohort from the HTLV-1 endemic region, Jamaica, consisted of seronegative controls, asymptomatic carriers (ACs), ATL, and HAM/TSP patients. This
ex vivo
analyses included two major subsets of blood DCs, myeloid and plasmacytoid (mDCs and pDCs, respectively). The comparative analyses of results demonstrated a decreased pDC frequency in both ATL and HAM/TSP patients as compared to ACs and seronegative controls. Similarly, CD86 expression on both mDCs and pDCs was significantly higher in HAM/TSP (but not ATL) patients compared to ACs. Interestingly, HLA-DR expression was significantly lower on pDCs of patients as compared to carriers; however, for mDCs, only the HAM/TSP group had significantly lower expression of HLA-DR. Unlike HAM/TSP individuals, ATL individuals had higher HLA-ABC expression on mDCs compared to ACs. Finally, both mDCs and pDCs of HAM/TSP patients had significantly higher expression of the programmed death ligand 1 (PD-L1) compared to ACs. Overall, this study suggests that DCs exhibit a differential phenotypic and functional profile between patients (ATL and HAM/TSP) and carriers of HTLV-1 and could provide an important tool for understanding HTLV-1 immunopathogenesis during infection and disease.
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Details
- Title
- An Altered Maturation and Adhesion Phenotype of Dendritic Cells in Diseased Individuals Compared to Asymptomatic Carriers of Human T Cell Leukemia Virus Type 1
- Creators
- Sharrón L Manuel - Department of Microbiology and Immunology, and the Drexel Institute for Biotechnology and VirologyMohit Sehgal - Department of Microbiology and Immunology, and the Drexel Institute for Biotechnology and VirologyZafar K Khan - Department of Microbiology and Immunology, and the Drexel Institute for Biotechnology and VirologyJames J Goedert - , Division of Cancer Epidemiology and Genetics, Bethesda, MarylandMichael R Betts - Department of Microbiology and ImmunologyPooja Jain - Department of Microbiology and Immunology, and the Drexel Institute for Biotechnology and Virology
- Publication Details
- AIDS research and human retroviruses, v 29(9), pp 1273-1285
- Publisher
- Mary Ann Liebert, Inc; 140 Huguenot Street, 3rd FloorNew Rochelle, NY 10801USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000323372600015
- Scopus ID
- 2-s2.0-84882990034
- Other Identifier
- 991014878292304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases
- Virology