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Accepted (AM)Open Access (License Unspecified), Open
Journal article
An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth
Journal of Visualized Experiments, v 2021(175)
2021
PMID: 34633392
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Brain metastasis is a serious consequence of breast cancer for women as these tumors are difficult to treat and are associated with poor clinical outcomes. Preclinical mouse models of breast cancer brain metastatic (BCBM) growth are useful but are expensive, and it is difficult to track live cells and tumor cell invasion within the brain parenchyma. Presented here is a protocol for ex vivo brain slice cultures from xenografted mice containing intracranially injected breast cancer brain-seeking clonal sublines. MDA-MB-231BR luciferase tagged cells were injected intracranially into the brains of Nu/Nu female mice, and following tumor formation, the brains were isolated, sliced, and cultured ex vivo. The tumor slices were imaged to identify tumor cells expressing luciferase and monitor their proliferation and invasion in the brain parenchyma for up to 10 days. Further, the protocol describes the use of time-lapse microscopy to image the growth and invasive behavior of the tumor cells following treatment with ionizing radiation or chemotherapy. The response of tumor cells to treatments can be visualized by live-imaging microscopy, measuring bioluminescence intensity, and performing histology on the brain slice containing BCBM cells. Thus, this ex vivo slice model may be a useful platform for rapid testing of novel therapeutic agents alone or in combination with radiation to identify drugs personalized to target an individual patient's breast cancer brain metastatic growth within the brain microenvironment.
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Details
- Title
- An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth
- Creators
- Lorela Ciraku - Drexel UniversityRebecca A. Moeller - Drexel UniversityEmily M. Esquea - Drexel UniversityWiktoria A. Gocal - Drexel UniversityEdward J. Hartsough - Thomas Jefferson UniversityNicole L. Simone - Thomas Jefferson UniversityJoshua G. Jackson - Drexel UniversityMauricio J. Reginato - Thomas Jefferson University
- Publication Details
- Journal of Visualized Experiments, v 2021(175)
- Publisher
- MyJove Corporation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Pharmacology and Physiology
- Web of Science ID
- WOS:000708102100006
- Scopus ID
- 2-s2.0-85118160789
- Other Identifier
- 991019169707104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Oncology