Journal article
An Expanded Role for HLA Genes: HLA-B Encodes a microRNA that Regulates IgA and Other Immune Response Transcripts
Frontiers in immunology, v 8(MAY), pp 583-583
19 May 2017
PMID: 28579988
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We describe a novel functional role for the
HLA-B
locus mediated by its intron-encoded microRNA (miRNA), miR-6891-5p. We show that
in vitro
inhibition of miR-6891-5p impacts the expression of nearly 200 transcripts within the B-lymphoblastoid cell line (B-LCL) COX, affecting a large number of metabolic pathways, including various immune response networks. The top affected transcripts following miR-6891-5p inhibition are those encoding the heavy chain of IgA. We identified a conserved miR-6891-5p target site on the 3′UTR of both immunoglobulin heavy chain alpha 1 and 2 (
IGHA1
and
IGHA2
) transcripts and demonstrated that this miRNA modulates the expression of
IGHA1
and
IGHA2
. B-LCLs from IgA-deficient patients expressed significantly elevated levels of miR-6891-5p when compared with unaffected family members. Upon inhibition of miR-6891-5p, IgA mRNA expression levels were increased, and IgA secretion was restored in the B-LCL of an IgA-deficient patient. These findings indicate that miR-6891-5p regulates
IGHA1
and
IGHA2
gene expression at the posttranscriptional level and suggest that increase in miR-6891-5p levels may contribute to the etiology of selective IgA deficiency.
Metrics
Details
- Title
- An Expanded Role for HLA Genes: HLA-B Encodes a microRNA that Regulates IgA and Other Immune Response Transcripts
- Creators
- Nilesh Chitnis - Children's Hospital of PhiladelphiaPeter M. Clark - Children's Hospital of PhiladelphiaMalek Kamoun - University of PennsylvaniaCatherine Stolle - Children's Hospital of PhiladelphiaF. Brad Johnson - , , , ,Dimitri S. Monos - University of Pennsylvania
- Publication Details
- Frontiers in immunology, v 8(MAY), pp 583-583
- Publisher
- Frontiers Media S.A
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000401585500001
- Scopus ID
- 2-s2.0-85020032073
- Other Identifier
- 991019356343404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Immunology