Journal article
An Integrated, Real-Time Convective PCR System for Isolation, Amplification, and Detection of Nucleic Acids
Chemosensors, v 10(7)
11 Jul 2022
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Convective PCR (CPCR) can perform rapid nucleic acid amplification by inducing thermal convection to continuously, cyclically driving reagent between different zones of the reactor for spatially separate melting, annealing, and extending in a capillary tube with constant heating temperatures at different locations. CPCR is promoted by incorporating an FTA membrane filter into the capillary tube, which constructs a single convective PCR reactor for both sample preparation and amplification. To simplify fluid control in sample preparation, lysed sample or wash buffer is driven through the membrane filter through centrifugation. A movable resistance heater is used to heat the capillary tube for amplification, and meanwhile, a smartphone camera is adopted to monitor in situ fluorescence signal from the reaction. Different from other existing CPCR systems with the described simple, easy-to-use, integrated, real-time microfluidic CPCR system, rapid nucleic acid analysis can be performed from sample to answer. A couple of critical issues, including wash scheme and reaction temperature, are analyzed for optimized system performance. It is demonstrated that influenza A virus with the reasonable concentration down to 1.0 TCID50/mL can be successfully detected by the integrated microfluidic system within 45 min.
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Details
- Title
- An Integrated, Real-Time Convective PCR System for Isolation, Amplification, and Detection of Nucleic Acids
- Creators
- Guijun Miao - Beijing University of Chemical TechnologyMeng Guo - Beijing University of Chemical TechnologyKe Li - Beijing Wantai Biological Pharmacy (China)Xiangzhong Ye - Beijing Wantai Biological Pharmacy (China)Michael G. Mauk - Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USAShengxiang Ge - Xiamen UniversityNingshao Xia - Xiamen UniversityDuli Yu - Beijing Advanced Sciences and Innovation CenterXianbo Qiu - Beijing University of Chemical Technology
- Publication Details
- Chemosensors, v 10(7)
- Publisher
- Mdpi
- Number of pages
- 16
- Grant note
- 81871505; 81371711 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) buctylkjcx06 / Beijing University of Chemical Technology XK1802-4; PYBZ1830; PT1908 / Fundamental Research Funds for the Central Universities 2018ZX10732101001-009 / National Science and Technology Major Project
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000833128000001
- Scopus ID
- 2-s2.0-85136139525
- Other Identifier
- 991020623759304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical
- Electrochemistry
- Instruments & Instrumentation