Journal article
An efficacy-dependent effect of cardiac overexpression of beta(2)-adrenoceptor on ligand affinity in transgenic mice
Molecular pharmacology, v 52(2)
01 Aug 1997
PMID: 9271340
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Abstract
In previous studies, it was shown that the overexpression of beta(2)-adrenoceptor (beta(2)AR) in the hearts of transgenic mice (Tg) leads to agonist-independent activation of adenylate cyclase and enhanced myocardia[ function. Here, we measured the physical coupling of beta(2)AR and G(s) by evaluating the coimmunoprecipitation of beta(2)AR and G(s) and the ligand binding properties of beta(2)AR in the hearts of Tg mice to investigate the details of the interaction among ligand, receptor, and G protein. The following results were obtained: (i) coimmunoprecipitation of beta(2)AR and G(s) was increased in the absence of agonist in Tg mice compared with the control animals. This demonstrates directly the increased interaction between unliganded beta(2)AR and G(s), which is consistent with increased background cAMP production and cardiac function in the hearts of Tg mice. (ii) Guanosine-5'-(beta,gamma-imido)triphosphate abolished the association of beta(2)AR/G(s) in the immunoprecipitate. (iii) The affinities for ligands that show agonist (isoproterenol, clenbuterol, and dobutamine), neutral antagonist (alprenolol and timolol), and negative antagonist (propranolol and ICI 118551) activities in this experimental system were increased, not changed and decreased, respectively, in Tg mice compared with the controls. (iv) This efficacy-dependent alteration in ligand affinities was still observed in the presence of a guanosine-5'-(beta,gamma-imido)triphosphate concentration that abolishes beta(2)AR/G(s) coupling. This suggests that the altered beta(2)AR binding affinities in Tg mice are not due to the increased interaction between beta(2)AR and G(s). These data cannot be explained by using ternary, quinternary, two-state extended ternary, or cubic ternary complex models. We therefore discuss the results using a ''two-state polymerization model'' that includes an isomerization step for the conversion of receptor between an inactive and an active form (denoted as R and R*, respectively) and a polymerization of the active state (R*(n)). The simplest form of this model (i.e., noncooperative dimerization of the receptor) is found to be consistent with the experimental data.
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Details
- Title
- An efficacy-dependent effect of cardiac overexpression of beta(2)-adrenoceptor on ligand affinity in transgenic mice
- Creators
- H GurdalR A BondM D JohnsonE FriedmanH O Onaran
- Publication Details
- Molecular pharmacology, v 52(2)
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1997XQ98400002
- Scopus ID
- 2-s2.0-0242701298
- Other Identifier
- 991019168296004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Pharmacology & Pharmacy