Journal article
An ice-templated, linearly aligned chitosan-alginate scaffold for neural tissue engineering
Journal of biomedical materials research. Part A, v 101(12), pp 3493-3503
01 Dec 2013
PMID: 23596011
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Several strategies have been investigated to enhance axonal regeneration after spinal cord injury, however, the resulting growth can be random and disorganized. Bioengineered scaffolds provide a physical substrate for guidance of regenerating axons towards their targets, and can be produced by freeze casting. This technique involves the controlled directional solidification of an aqueous solution or suspension, resulting in a linearly aligned porous structure caused by ice templating. In this study, freeze casting was used to fabricate porous chitosan-alginate (C/A) scaffolds with longitudinally oriented channels. Chick dorsal root ganglia explants adhered to and extended neurites through the scaffold in parallel alignment with the channel direction. Surface adsorption of a polycation and laminin promoted significantly longer neurite growth than the uncoated scaffold (poly-L-ornithine + Laminin = 793.2 +/- 187.2 mu m; poly-L-lysine + Laminin = 768.7 +/- 241.2 mu m; uncoated scaffold = 22.52 +/- 50.14 mu m) (P < 0.001). The elastic modulus of the hydrated scaffold was determined to be 5.08 +/- 0.61 kPa, comparable to reported spinal cord values. The present data suggested that this C/A scaffold is a promising candidate for use as a nerve guidance scaffold, because of its ability to support neuronal attachment and the linearly aligned growth of DRG neurites. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3493-3503, 2013.
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Details
- Title
- An ice-templated, linearly aligned chitosan-alginate scaffold for neural tissue engineering
- Creators
- Nicola L. Francis - Drexel UniversityPhilipp M. Hunger - Department of Materials Science and EngineeringDrexel University3141 Chestnut StreetPhiladelphiaAmalie E. Donius - Department of Materials Science and EngineeringDrexel University3141 Chestnut StreetPhiladelphiaBenjamin W. Riblett - Department of Materials Science and EngineeringDrexel University3141 Chestnut StreetPhiladelphiaAntonios Zavaliangos - Drexel UniversityUlrike G. K. Wegst - Dartmouth CollegeMargaret A. Wheatley - Drexel University
- Publication Details
- Journal of biomedical materials research. Part A, v 101(12), pp 3493-3503
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- 1R01DE015633 / NIDCR NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) R01DE015633 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000326121000015
- Scopus ID
- 2-s2.0-84886726398
- Other Identifier
- 991019169697304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials