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Journal article
An inducible propane monooxygenase is responsible for N-nitrosodimethylamine degradation by Rhodococcus sp. strain RHA1
Applied and environmental microbiology, v 73(21), pp 6930-6938
01 Nov 2007
PMID: 17873074
Abstract
Rhodococci are common soil heterotrophs that possess diverse functional enzymatic activities with economic and ecological significance. In this study, the correlation between gene expression and biological removal of the water contaminant N-nitrosodimethylamine (NDMA) is explored. NDMA is a hydrophilic, potent carcinogen that has gained recent notoriety due to its environmental persistence and emergence as a widespread micropollutant in the subsurface environment. In this study, we demonstrate that Rhodococcus sp. strain RHA1 can constitutively degrade NDMA and that activity toward this compound is enhanced by approximately 500-fold after growth on propane. Transcriptomic analysis of RHA1 and reverse transcriptase quantitative PCR assays demonstrate that growth on propane elicits the upregulation of gene clusters associated with (i) the oxidation of propane and (ii) the oxidation of substituted benzenes. Deletion mutagenesis of prmA, the gene encoding the large hydroxylase component of propane monooxygenase, abolished both growth on propane and removal of NDMA. These results demonstrate that propane monooxygenase is responsible for NDMA degradation by RHA1 and explain the enhanced cometabolic degradation of NDMA in the presence of propane.
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Details
- Title
- An inducible propane monooxygenase is responsible for N-nitrosodimethylamine degradation by Rhodococcus sp. strain RHA1
- Creators
- Jonathan O Sharp - Colorado School of MinesChristopher M Sales - Drexel University, C. and J. Nyheim Plasma InstituteJustin C LeBlanc - University of British ColumbiaJie Liu - University of British ColumbiaThomas K Wood - Texas A&M UniversityLindsay D Eltis - University of British ColumbiaWilliam W Mohn - University of British ColumbiaLisa Alvarez-Cohen - University of California, Berkeley
- Publication Details
- Applied and environmental microbiology, v 73(21), pp 6930-6938
- Publisher
- American Society for Microbiology
- Grant note
- P42 ES004705 / NIEHS NIH HHS 3 P42 ES004705-19S1 / NIEHS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- C. and J. Nyheim Plasma Institute; Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:000250700600027
- Scopus ID
- 2-s2.0-35949001312
- Other Identifier
- 991021895795904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology