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Journal article
An integrated microchannel biosensor platform to analyse low density lactate metabolism in HepG2 cells in vitro
RSC advances, v 9(16), pp 9006-9013
15 Mar 2019
PMID: 35517697
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this study, we developed an electrochemical microchannel biosensor platform to analyse lactate metabolism in cells. This biosensor platform was fabricated by photolithography, thin-film deposition and microfluidic technology. A kind of functional biomaterial was prepared by mixing lactate oxidase, single-walled carbon nanotubes and chitosan, and platinum as working and blank electrodes of the biosensor was modified by a thin Prussian blue layer. The lactate biosensor was obtained by dropping functional biomaterials on the electrode. The results demonstrated that the sensitivity of the electrochemical biosensor was up to 567 nA mM
mm
and the limit of detection was 4.5 μM (
Ag/AgCl as the counter/reference electrode). The biosensor used to quantitatively detect metabolic lactate concentrations in HepG2 cells cultured with cancer drugs showed high sensitivity, selectivity and stability, and has potential applications in organ-on-a-chip and tissue engineering technologies, which typically involve low concentrations of metabolites.
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Details
- Title
- An integrated microchannel biosensor platform to analyse low density lactate metabolism in HepG2 cells in vitro
- Creators
- Shengli Mi - Tsinghua UniversityJingjing Xia - University Town of ShenzhenYuanyuan Xu - University Town of ShenzhenZhichang Du - University Town of ShenzhenWei Sun - University Town of Shenzhen
- Publication Details
- RSC advances, v 9(16), pp 9006-9013
- Publisher
- Royal Society of Chemistry
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000463949500023
- Scopus ID
- 2-s2.0-85063329375
- Other Identifier
- 991019167475504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary