Journal article
An isobutyronitrile-induced bienzymatic system of Alcaligenes sp MTCC 10674 and its application in the synthesis of alpha-hydroxyisobutyric acid
Bioprocess and biosystems engineering, v 36(5), pp 613-625
01 May 2013
PMID: 22945851
Abstract
Alcaligenes sp. MTCC 10674 was isolated as acetone cyanohydrin hydrolyzing bacterium from soil of orchid gardens of Himachal Pradesh. Acetone cyanohydrin hydrolyzing activity of this organism comprised nitrile hydratase and amidase activities. It exhibited higher substrate specificity towards aliphatic hydroxynitrile (acetone cyanohydrin) in comparison to arylaliphatic hydroxynitrile. Isobutyronitrile (40 mM) acted as a carbon source as well as inducer for growth of Alcaligenes sp. MTCC 10674 and expression of acetone cyanohydrin hydrolyzing activity. Optimization of culture condition using response surface methodology increased acetone cyanohydrin hydrolyzing activity by 1.3-fold, while inducer mediation approach increased the activity by 1.2-fold. The half life of this enzyme was 25 h at 15 A degrees C. V (max) and K (m) value for acetone cyanohydrin hydrolyzing enzyme was 0.71 mu mol mg(-1) min(-1) and 14.3 mM, when acetone cyanohydrin was used as substrate. Acetone cyanohydrin hydrolyzing enzyme encountered product inhibition and IC50 and K (i) value were calculated to be 28 and 10.2 mM, respectively, when product alpha-hydroxyisobutyric acid was added in the reaction. Under optimized reaction conditions at 40 ml fed batch scale, 3 mg dcw ml (-) resting cells of Alcaligenes sp. MTCC 10674 fully converted 0.33 M acetone cyanohydrin into alpha-hydroxyisobutyric acid (1.02 g) in 6 h 40 min. The characterization of acetone cyanohydrins hydrolyzing activity revealed that it comprises bienzymatic nitrile hydrolyzing system, i.e. nitrile hydratase and amidase for the production of alpha-hydroxyisobutyric acid from acetone cyanohydrin and maximum 70 % yield is being reported for the first time.
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Details
- Title
- An isobutyronitrile-induced bienzymatic system of Alcaligenes sp MTCC 10674 and its application in the synthesis of alpha-hydroxyisobutyric acid
- Creators
- S. K. Bhatia - Himachal Pradesh UniversityP. K. Mehta - Himachal Pradesh UniversityR. K. Bhatia - Himachal Pradesh UniversityT. C. Bhalla - Himachal Pradesh University
- Publication Details
- Bioprocess and biosystems engineering, v 36(5), pp 613-625
- Publisher
- Springer Nature
- Number of pages
- 13
- Grant note
- Department of Biotechnology University Grant Commission, India; University Grants Commission, India
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000318286500011
- Scopus ID
- 2-s2.0-84877920445
- Other Identifier
- 991021229894204721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Engineering, Chemical