Journal article
Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbes
Biochemical and biophysical research communications, v 314(4), pp 957-963
20 Feb 2004
PMID: 14751225
Abstract
Degradation of TNT by bovine rumen fluid, a novel source of anaerobic microbes, was investigated. Whole rumen fluid contents were spiked with TNT and incubated for a 24
h time period. Supernatant samples taken at 0, 1, 2, 4, and 24
h were analyzed by reverse-phase HPLC with diode array detection. Within 1
h, TNT was not detectable and reduction products of TNT including 2-hydroxyl-amino-4,6-dinitrotoluene, 4-hydroxylamino-2,6-dinitrotoluene, and 4-amino-2,6-dinitrotoluene were present with smaller amounts of diamino-nitrotoluenes. Within 2
h, only the diamino and dihydroxyamino-nitrotoluene products remained. After 4
h, 2,4-diamino-6-nitrotoluene and 2,4-dihydroxyamino-6-nitrotoluene were the only known molecular species left. At 24
h known UV absorbing metabolites were no longer detected, suggesting further transformation such as complete reduction to triaminotoluene or destruction of the aromatic ring of TNT may have occurred. TNT was not transformed at 24
h in autoclaved and buffered controls. This study presents the first direct evidence of biodegradation of TNT by ruminal microbes.
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Details
- Title
- Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbes
- Creators
- Thomas J Fleischmann - Oregon State UniversityKaren C Walker - Oregon State UniversityJim C Spain - United States Air Force Research LaboratoryJoseph B Hughes - Rice UniversityA Morrie Craig - Oregon State University
- Publication Details
- Biochemical and biophysical research communications, v 314(4), pp 957-963
- Publisher
- Elsevier
- Number of pages
- 7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:000188798100006
- Scopus ID
- 2-s2.0-0942278962
- Other Identifier
- 991021931900504721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics