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Analysis and HPLC fractionation of urine from patients with cystic fibrosis, chronic lung diseases and normal controls
Journal article   Peer reviewed

Analysis and HPLC fractionation of urine from patients with cystic fibrosis, chronic lung diseases and normal controls

Terese M. Guman-Wignot, Jay Kaufman, Douglas S. Holsclaw, Irvin R. Schmoyer and Jack Alhadeff
Clinical biochemistry, v 22(5), pp 377-383
1989
PMID: 2805341

Abstract

carbohydrate creatinine sialic acid size exclusion chromatography
The amounts of creatinine, protein, carbohydrate and sialic acid in the urine of 19 patients with cystic fibrosis (CF), 12 normal controls and 11 pathological controls with chronic lung disease have been determined. The mean creatinine excretion levels of the total CF group as well as the CF subgroups are significantly decreased when compared to normal controls but comparable to pathological controls. Mean urinary protein levels appear to be increased in patients with CF compared to normal controls and pathological controls but the increased levels resulted from factors ( e.g. presence of diabetes mellitus) other than CF. No significant differences were found in amounts of total carbohydrate and sialic acid in urine and fractionated urinary preparations for the total group of nondiabetic patients with CF when compared to both normal and pathological controls. HPLC fractionation of low M, (<10,000 Daltons) urinary preparations indicated the presence of an unknown peak in all of the antibiotic-treated CF patients, 43% of CF patients on low or no medication, 17% of the normal controls and 9% of the pathological controls. The present results illustrate the importance of including appropriate pathological controls and dividing patients with CF into subgroups according to clinical factors and types of therapy employed.

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Collaboration types
Domestic collaboration
Web of Science research areas
Medical Laboratory Technology
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