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Analysis of Common Single Nucleotide Polymorphisms in Complex Regional Pain Syndrome: Genome Wide Association Study Approach and Pooled DNA Strategy
Journal article   Open access   Peer reviewed

Analysis of Common Single Nucleotide Polymorphisms in Complex Regional Pain Syndrome: Genome Wide Association Study Approach and Pooled DNA Strategy

Piotr K Janicki, Guillermo M Alexander, Jill Eckert, Marek Postula and Robert J Schwartzman
Pain medicine (Malden, Mass.), v 17(12), pp 2344-2352
Dec 2016
PMID: 28025368
url
https://doi.org/10.1093/pm/pnw133View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

Adolescent Adult Aged Cohort Studies Complex Regional Pain Syndromes - genetics DNA - analysis DNA - genetics Female Genome-Wide Association Study Genotype Humans Male Middle Aged Oligonucleotide Array Sequence Analysis Polymorphism, Single Nucleotide Young Adult
The objective of this study was to use a genome-wide association (GWAS) approach and pooled DNA strategy to search for new genomic loci associated with complex regional pain syndrome (CRPS). The study cohort consisted of 230 patients with established diagnosis of CRPS. The control group consisted of 230 age- and gender-matched subjects without chronic pain. We tested the association of common single nucleotide polymorphisms (SNPs), genotyped using a high-density microarray platform, with CRPS phenotype. This was followed by individual genotyping of the most significant SNPs identified in the microarray genomic scan, in both original discovery (N = 115) and independent verification (N = 115) groups of patients with CRPS, as well as in the appropriate matched control subjects. The results of our study provide no support for the initial hypothesis of the existence of an association between any investigated genomic targets (including GWAS for all genomic loci available on the microarray, and focused scan of the HLA locus on chromosome 6) and CRPS phenotype. Despite the fact that we interrogated about 83% of all of common SNPs in the human genome, we did not find evidence that any of the investigated common SNPs may be associated with CRPS phenotype.

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Collaboration types
Domestic collaboration
Web of Science research areas
Anesthesiology
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