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Analysis of gene expression in carbon tetrachloride-treated rat livers using a novel bioarray technology
Journal article   Open access   Peer reviewed

Analysis of gene expression in carbon tetrachloride-treated rat livers using a novel bioarray technology

M B Young, M R DiSilvestro, T J Sendera, J Freund, A Kriete and S R Magnuson
The pharmacogenomics journal, v 3(1)
10 Mar 2003
PMID: 12629582
url
https://doi.org/10.1038/sj.tpj.6500147View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

Carbon Tetrachloride - pharmacology Gene Expression Regulation - genetics Oligonucleotide Array Sequence Analysis - methods Liver - metabolism Gene Expression Regulation - physiology Rats Male Up-Regulation - genetics Rats, Sprague-Dawley Gene Expression Regulation - drug effects Up-Regulation - drug effects Animals Liver - drug effects Up-Regulation - physiology
The present study successfully utilizes a new ADME Rat Expression Bioarray, containing 1040 metabolism- and toxicology-linked genes, to monitor gene expression from the livers of rats treated with carbon tetrachloride (CCl(4)). Histopathological analysis, hierarchical clustering methods, and gene expression profiling are compared between the control and CCl(4)-treated animals. A total of 44 transcripts were found to be altered in response to the hepatotoxin, 19 of which were upregulated and 25 were downregulated. Some of these gene expression changes were expected and concurred with previously published data while others were novel findings.

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16 citations in Scopus

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Web of Science research areas
Genetics & Heredity
Pharmacology & Pharmacy
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