Journal article
Analysis of microRNA expression and micronuclei frequency in workers exposed to vinyl chloride monomer in China
Epigenomics, v 9(8), pp 1093-1104
Aug 2017
PMID: 28749179
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To identify differently expressed miRNAs associated with vinyl chloride monomer (VCM) and micronuclei (MN) frequency.
In discovery stage, we used microarray to detect miRNAs expression in peripheral blood lymphocytes between six low and six high VCM-exposed workers grouped by medium cumulative exposure dose. Then we validated four miRNAs using real-time quantitative reverse transcription PCR (qRT-PCR) and detected the micronuclei frequencies using cytokinesis-block micronucleus assay in 94 VCM-exposed workers and 53 healthy control subjects.
We found eight miRNAs significantly downregulated and seven miRNAs upregulated (|Fold Change| >2; p < 0.05) in the high-exposure group through microarray. We validate that miR-222-3p, miR-146a-5p and miR-151a-5p were downregulated, while miR-22-3p was upregulated in VCM-exposed group (all p < 0.01). Furthermore, we found that expression of miR-22-3p was upregulated in the high micronuclei (MN) frequency subjects. In conclusion, our study suggested that these four miRNAs could be biomarkers of VCM exposure, and moreover miR-22-3p was correlated with MN frequency.
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Details
- Title
- Analysis of microRNA expression and micronuclei frequency in workers exposed to vinyl chloride monomer in China
- Creators
- Nan-Nan Feng - Department of Occupational Health & Toxicology, School of Public Health, Fudan University, & Key Laboratory of Public Health & Safety of Ministry of Education of China, Shanghai, 200032, China.Yan Fang - Department of Occupational Health & Toxicology, School of Public Health, Fudan University, & Key Laboratory of Public Health & Safety of Ministry of Education of China, Shanghai, 200032, China.Ya-Nan Zhang - Department of Occupational Health & Toxicology, School of Public Health, Fudan University, & Key Laboratory of Public Health & Safety of Ministry of Education of China, Shanghai, 200032, China.Xiao-Wen Xu - Department of Occupational Health & Toxicology, School of Public Health, Fudan University, & Key Laboratory of Public Health & Safety of Ministry of Education of China, Shanghai, 200032, China.Yong Li - Department of Occupational Health & Toxicology, School of Public Health, Fudan University, & Key Laboratory of Public Health & Safety of Ministry of Education of China, Shanghai, 200032, China.Jin-Wei Wang - Department of Occupational Health & Toxicology, School of Public Health, Fudan University, & Key Laboratory of Public Health & Safety of Ministry of Education of China, Shanghai, 200032, China.Yong-Liang Li - Department of Occupational Health & Toxicology, School of Public Health, Fudan University, & Key Laboratory of Public Health & Safety of Ministry of Education of China, Shanghai, 200032, China.Paul Brandt-Rauf - University of Illinois ChicagoZhao-Lin Xia - Department of Occupational Health & Toxicology, School of Public Health, Fudan University, & Key Laboratory of Public Health & Safety of Ministry of Education of China, Shanghai, 200032, China.
- Publication Details
- Epigenomics, v 9(8), pp 1093-1104
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000408645600005
- Scopus ID
- 2-s2.0-85028830759
- Other Identifier
- 991019176801204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Genetics & Heredity