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Analysis of the cytolytic T-lymphocyte response to herpes simplex virus type 1 glycoprotein B during primary and secondary infection
Journal article   Open access   Peer reviewed

Analysis of the cytolytic T-lymphocyte response to herpes simplex virus type 1 glycoprotein B during primary and secondary infection

C T Nugent, R M Wolcott, R Chervenak and S R Jennings
Journal of virology, v 68(11), pp 7644-7648
Nov 1994
PMID: 7933156
url
https://doi.org/10.1128/JVI.68.11.7644-7648.1994View
Published, Version of Record (VoR) Open

Abstract

The immune response to herpes simplex virus type 1 (HSV-1) infection in C57BL/6 mice includes a population of major histocompatibility complex class I-restricted cytolytic T lymphocytes (CTL) that recognize the structural glycoprotein gB. To gain insight into the importance of this CTL subpopulation in vivo, gB-specific CTL present in the regional lymph nodes after a primary infection and after a reinfection of convalescent animals were analyzed. In a primary infection, gB-specific CTL precursors (CTLp) that recognized either a cell line constitutively expressing gB or cells pulsed with the optimal Kb-restricted gB epitope 498SSIEFARL505 were present at an estimated frequency of 1/12,000 compared with a frequency of 1/3,000 for CTLp which recognized cells infected with HSV-1 itself. In convalescent mice responding to reinfection, HSV-specific CTLp were present at an estimated frequency of 1/4,000 to 1/14,000. However, gB-specific CTLp could not be detected at this site. These findings suggest that CTL specific for an immunodominant epitope contribute substantially to the primary response but may not be a component of the HSV-specific CTL population that responds rapidly to reinfection in vivo.

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