Journal article
Analytical affinity chromatography in studies of molecular recognition in biology: A review
Journal of chromatography. Biomedical applications, v 376(C), pp 11-32
1986
PMID: 3519631
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Measuring macromolecular and cellular interactions remains central to the study of recognition in biology and its application in biotechnology. Analytical affinity chromatography provides a versatile methodology to detect and quantitate such interactions. Both zonal and frontal elution approaches have been developed, essentially in parallel, for analytical affinity chromatography. A close quantitative relatedness of chromatographically obtained equilibrium constants and analogous constants determined fully in solution has been found for a growing number of proteins. This consistently observed correlation has formed the basis for extending theoretical treatments in order to evaluate not only monovalent molecular systems of varying types but also multivalently interacting macromolecules, including those which exhibit cooperativity, The potential to measure chemical rate constants by affinity chromatography also has been recognized, and experimental tests of the available theory are being made. As a micromethod, the quantitative use of affinity chromatography has important applicability for biochemical analysis of an increasing array of biologically active molecules being discovered and isolated but available in only relatively small amounts. Analytical affinity chromatography thus provides a means to use matrix-mobile interactant systems to study mechanisms of biomolecular interactions and therein to attain an understanding of such interactions which often is not easily achieved by solution methods alone.
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Details
- Title
- Analytical affinity chromatography in studies of molecular recognition in biology: A review
- Creators
- Irwin M. Chaiken - National Institute of Diabetes and Digestive and Kidney Diseases
- Publication Details
- Journal of chromatography. Biomedical applications, v 376(C), pp 11-32
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Drexel University
- Web of Science ID
- WOS:A1986A985400004
- Scopus ID
- 2-s2.0-0023046684
- Other Identifier
- 991019520715704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical