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Analytical performance evaluation of the scanning capillary tube viscometer for measurement of whole blood viscosity
Journal article   Peer reviewed

Analytical performance evaluation of the scanning capillary tube viscometer for measurement of whole blood viscosity

Hanah Kim, Young I. Cho, Dong-Hwan Lee, Chul-Min Park, Hee-Won Moon, Mina Hur, Jin Q. Kim and Yeo-Min Yun
Clinical biochemistry, v 46(01-02), pp 139-142
Jan 2013
PMID: 23099199

Abstract

Analytical performance Capillary tube viscometer Evaluation Viscometer Whole blood viscosity
Whole blood viscosity (WBV) is the resistance of blood flow in blood vessels. Increased WBV may be a cardiovascular risk factor. The proper screening of WBV can help the early detection of cardiovascular disease. We investigated the performance of a new scanning capillary tube viscometer (SCTV) for the measurement of WBV. We evaluated the total precision of the SCTV for 20days using three control viscosity materials, and the within-day precision with the whole blood samples of three different individuals. For the linearity evaluation, serial dilutions of a high concentration standard material were used. For the method comparison, the results of the SCTV method were compared to those of Brookfield rotating viscometer on 227 subjects. The SCTV had good within-run and total-run coefficient of variant (CV)s at low-, medium-, and high-concentration samples, at shear rates of 1 and 300s−1. The within-day CVs with the three human blood samples were 6.3%, 3.7% and 3.8% at a shear rate of 1s−1, and 3.2%, 3.0% and 4.1% at a shear rate of 300s−1. The SCTV method showed an excellent linearity in the range of 84.9 to 558.2 milliPoise (mP) and 28.8 to 71.0 mP at shear rates of 1 and 300s−1, respectively. For the comparison study, the SCTV and the rotating viscometer showed comparable results. The SCTV showed a stable analytical performance, and was comparable with the rotational viscometer. This new SCTV method can be used in the clinical laboratory for various needs. ► We investigated the performance of a new scanning capillary tube viscometer (SCTV) with the Clinical Laboratory and Standards Institute (CLSI). ► The SCTV showed excellent within-run CVs, between-run, between-day, and total-run CVs at the low-, mid-, and high-concentration samples at high and low shear rate. ► The SCTV was linear over the entire range tested with good recovery at high and low shear rate. ► The WBV using the SCVT showed comparable results with the Brookfield viscometer.

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Medical Laboratory Technology
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