Journal article
Anchoring and synaptic stability of PSD-95 is driven by ephrin-B3
Nature neuroscience, v 18(11), pp 1594-1605
01 Nov 2015
PMID: 26479588
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Organization of signaling complexes at excitatory synapses by membrane-associated guanylate kinase (MAGUK) proteins regulates synapse development, plasticity, senescence and disease. Post-translational modification of MAGUK family proteins can drive their membrane localization, yet it is unclear how these intracellular proteins are targeted to sites of synaptic contact. Here we show using super-resolution imaging, biochemical approaches and in vivo models that the trans-synaptic organizing protein ephrin-B3 controls the synaptic localization and stability of PSD-95 and links these events to changes in neuronal activity via negative regulation of a newly identified mitogen-associated protein kinase (MAPK)-dependent phosphorylation site on ephrin-B3, Ser332. Unphosphorylated ephrin-B3 was enriched at synapses, and interacted directly with and stabilized PSD-95 at synapses. Activity-induced phosphorylation of Ser332 dispersed ephrin-B3 from synapses, prevented the interaction with PSD-95 and enhanced the turnover of PSD-95. Thus, ephrin-B3 specifies the synaptic localization of PSD-95 and likely links the synaptic stability of PSD-95 to changes in neuronal activity.
Metrics
Details
- Title
- Anchoring and synaptic stability of PSD-95 is driven by ephrin-B3
- Creators
- Martin Hruska - Jefferson Hospital for NeuroscienceNathan T Henderson - Jefferson Hospital for NeuroscienceNan L Xia - Jefferson Hospital for NeuroscienceSylvain J Le Marchand - Drexel UniversityMatthew B Dalva - Jefferson Hospital for Neuroscience
- Publication Details
- Nature neuroscience, v 18(11), pp 1594-1605
- Publisher
- Springer Nature
- Number of pages
- 12
- Grant note
- R01 DA022727 / NIDA NIH HHS R01 MH073357 / NIMH NIH HHS R01 MH086425 / NIMH NIH HHS MH100093 / NIMH NIH HHS R01 MH100093 / NIMH NIH HHS MH086425 / NIMH NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Cell Imaging Center
- Web of Science ID
- WOS:000363830700017
- Scopus ID
- 2-s2.0-84945405591
- Other Identifier
- 991021892515504721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
Source: SDGs in the Output
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences