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Angiopoietin Level Trajectories in Toddlers With Severe Sepsis and Septic Shock and Their Effect on Capillary Endothelium
Journal article   Open access

Angiopoietin Level Trajectories in Toddlers With Severe Sepsis and Septic Shock and Their Effect on Capillary Endothelium

Richard W Pierce, Veronika Shabanova, Michael Canarie, Mathew Pinto, Yong Sing da Silva, Vineet Bhandari and John S Giuliano, Jr
Shock (Augusta, Ga.), v 51(3), pp 298-305
Mar 2019
DOI: https://doi.org/10.1097/SHK.0000000000001172
PMID: 30286031
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1097/shk.0000000000001172View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze),  Open

Abstract

Age Factors Angiopoietin-1 - blood Angiopoietin-2 - blood Capillaries - metabolism Capillaries - pathology Capillary Permeability Child, Preschool Endothelium, Vascular - metabolism Endothelium, Vascular - pathology Female Humans Infant Male Prospective Studies Severity of Illness Index Shock, Septic - blood Shock, Septic - pathology
Angiopoietins are postulated diagnostic biomarkers in children and adults with severe sepsis and septic shock. The diagnostic value of angiopoietins in children less than 5 years old has not been established, nor has their effect on permeability in the capillary microvasculature. We aim to determine if levels of angiopoietin-1 or -2 (angpt-1, -2) are diagnostic for severe sepsis/shock in young children and whether they affect the permeability of cultured human dermal microvascular endothelial cells (HDMEC). Prospective observational study of children < 5 years old. Patients were classified as non-systemic inflammatory response syndrome (SIRS), SIRS/sepsis and severe sepsis/septic shock. Tertiary care pediatric hospitals. Critically ill children. None. Plasma angpt-1 and -2 levels were measured with enzyme-linked immunoassays. Expression of angpt-2 in endothelial cells was assessed with quantitative polymerase chain reaction. Permeability changes in cultured HDMECs were assessed with transendothelial electrical resistance measurements. Angpt-1 levels were significantly higher in younger children compared with levels found in previous study of older children across disease severity (all P < 0.001). Angpt-2 was significantly higher in this cohort with severe sepsis/septic shock compared with children without SIRS and SIRS/sepsis (all P < 0.003). Angpt-2/1 ratio was also elevated in children with severe sepsis/septic shock but an order of magnitude less than older children (P < 0.02, P = 0.002). Angpt-1 and -2 did not affect basal HDMEC permeability or modulate leak in isolation or in the presence of tumor necrosis factor (TNF). Angpt-2 levels and the angpt-2/1 ratio are appropriate diagnostic biomarkers of severe sepsis/septic shock in children less than 5 years old. Neither angpt-1 nor -2 affects basal HDMEC permeability alone or modulates TNF induced capillary leak.

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11 citations in Web of Science
14 citations in Scopus

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UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

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Collaboration types
Domestic collaboration
Web of Science research areas
Critical Care Medicine
Hematology
Peripheral Vascular Disease
Surgery
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