Journal article
Anisotropy in Sickle Hemoglobin Fibers from Variations in Bending and Twist
Journal of molecular biology, v 357(5), pp 1422-1427
2006
PMID: 16490203
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have studied the variations of twist and bend in sickle hemoglobin fibers. We find that these variations are consistent with an origin in equilibrium thermal fluctuations, which allows us to estimate the bending and torsional rigidities and effective corresponding material moduli. We measure bending by electron microscopy of frozen hydrated fibers and find that the bending persistence length, a measure of the length of fiber required before it starts to be significantly bent due to thermal fluctuations, is 130
μm, somewhat shorter than that previously reported using light microscopy. The torsional persistence length, obtained by re-analysis of previously published experiments, is found to be only 2.5
μm. Strikingly this means that the corresponding torsional rigidity of the fibers is only 6×10
−27
J
m, much less than their bending rigidity of 5×10
−25
J
m. For (normal) isotropic materials, one would instead expect these to be similar. Thus, we present the first quantitative evidence of a very significant material anisotropy in sickle hemoglobin fibers, as might arise from the difference between axial and lateral contacts within the fiber. We suggest that the relative softness of the fiber with respect to twist deformation contributes to the metastability of HbS fibers: HbS double strands are twisted in the fiber but not in the equilibrium crystalline state. Our measurements inform a theoretical model of the thermodynamic stability of fibers that takes account of both bending and extension/compression of hemoglobin (double) strands within the fiber.
Metrics
Details
- Title
- Anisotropy in Sickle Hemoglobin Fibers from Variations in Bending and Twist
- Creators
- M.S Turner - Department of Physics, University of Warwick, Coventry CV4 7AL, UKR.W Briehl - Department of Physiology & Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USAJ.C Wang - Department of Physiology & Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USAF.A Ferrone - Department of Physics, Drexel University, Philadelphia, PA 19104, USAR Josephs - Department of Molecular Genetics & Cell Biology, University of Chicago, Chicago, IL 60637, USA
- Publication Details
- Journal of molecular biology, v 357(5), pp 1422-1427
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Physics
- Web of Science ID
- WOS:000236629400007
- Scopus ID
- 2-s2.0-33645087118
- Other Identifier
- 991014877708204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology