Journal article
Antibody-based immobilization of bioluminescent bacterial sensor cells
Talanta (Oxford), v 55(5), pp 1029-1038
2001
PMID: 18968453
Abstract
Whole-cell luminescent bioreporter sensors based on immobilized recombinant
Escherichia coli are described and evaluated. The sensors were prepared by glutaraldehyde-anchoring of nonspecific anti-
E. coli antibodies on aminosylilated gold or silica glass surfaces with subsequent attachment of the probe bacteria. We demonstrate the generality of the concept by attachment of several
E. coli strains that express luciferase in response to different physiological stress conditions including heat shock, DNA damage (SOS), fatty acid availability, peroxide and oxidative stress. The sensors can be used either as single- or multiple-use disposable sensing elements or for continuous operation. We show compatibility with optical fiber technology. Storage stability of the sensors exceeded 5 months with no measurable deterioration of the signal. Repeatability on exposure in successive days was <15%, as was sensor to sensor reproducibility. Sensitivity and detection limits of the immobilized cells were comparable to that of non-immobilized bacteria.
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Details
- Title
- Antibody-based immobilization of bioluminescent bacterial sensor cells
- Creators
- J.Rajan Premkumar - Division of Environmental Sciences, Fredy and Nadine Hermann Graduate School of Applied Science, The Hebrew University of Jerusalem, Jerusalem 91904, IsraelOvadia Lev - Division of Environmental Sciences, Fredy and Nadine Hermann Graduate School of Applied Science, The Hebrew University of Jerusalem, Jerusalem 91904, IsraelRobert S Marks - Department of Biotechnology Engineering, The Institute for Applied Biosciences, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, IsraelBoris Polyak - Department of Biotechnology Engineering, The Institute for Applied Biosciences, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, IsraelRachel Rosen - Division of Environmental Sciences, Fredy and Nadine Hermann Graduate School of Applied Science, The Hebrew University of Jerusalem, Jerusalem 91904, IsraelShimshon Belkin - Division of Environmental Sciences, Fredy and Nadine Hermann Graduate School of Applied Science, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
- Publication Details
- Talanta (Oxford), v 55(5), pp 1029-1038
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery
- Web of Science ID
- WOS:000172737900017
- Scopus ID
- 2-s2.0-0035856740
- Other Identifier
- 991014878499604721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical