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Journal article
Antimicrobial efficacy and wound-healing property of a topical ointment containing nitric-oxide-loaded zeolites
Journal of medical microbiology, v 63(2)
01 Feb 2014
PMID: 24196133
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Topical delivery of nitric oxide (NO) through a wound dressing has the potential to reduce wound infections and improve healing of acute and chronic wounds. This study characterized the antibacterial efficacy of an ointment containing NO-loaded, zinc-exchanged zeolite A that releases NO upon contact with water. The release rate of NO from the ointment was measured using a chemiluminescence detection system. Minimum bactericidal concentration assays were performed using five common wound pathogens, including Gram-negative bacteria (Escherichia coli and Acinetobacter baumannii), Gram-positive bacteria (Staphylococcus epidermidis and meticillin-resistant Staphylococcus aureus) and a fungus (Candida albicans). The time dependence of antimicrobial activity was characterized by performing log-reduction assays at four time points after 1-8 h ointment exposure. The cytotoxicity of the ointment after 24 h was assessed using cultured 3T3 fibroblast cells. Minimum microbicidal concentrations (MMCs) for bacterial organisms (5x10(7) c.f.u.) ranged from 50 to 100 mg ointment (ml media)(-1); the MMC for C. albicans (5x10(4) c.f.u.) was 50 mg ointment (ml media)(-1). Five to eight log reductions in bacterial viability and three log reductions in fungal viability were observed after 8 h exposure to NO zeolite ointment compared with untreated organisms. Fibroblasts remained viable after 24 h exposure to the same concentration of NO zeolite ointment as was used in antimicrobial tests. In parallel studies, full-thickness cutaneous wounds on Zucker obese rats healed faster than wounds treated with a control ointment. These data indicate that ointment containing NO-loaded zeolites could potentially be used as a broad-spectrum antimicrobial wound-healing dressing.
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Details
- Title
- Antimicrobial efficacy and wound-healing property of a topical ointment containing nitric-oxide-loaded zeolites
- Creators
- Michael Neidrauer - Zeomedix (United States)Utku K. Ercan - Drexel UniversityAparna Bhattacharyya - Drexel UniversityJoshua Samuels - Drexel UniversityJason Sedlak - Drexel UniversityRitika Trikha - Drexel UniversityKenneth A. Barbee - Drexel UniversityMichael S. Weingarten - Drexel UniversitySuresh G. Joshi - Drexel University
- Publication Details
- Journal of medical microbiology, v 63(2)
- Publisher
- SOC GENERAL MICROBIOLOGY
- Number of pages
- 7
- Grant note
- W81XWH-11-C-0031 / US Army Medical Research and Materiel Command; U.S. Army Medical Research & Materiel Command (USAMRMC) Zeomedix R13ES027302 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- MD (Doctor of Medicine) Program; School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000338478200006
- Scopus ID
- 2-s2.0-84892984035
- Other Identifier
- 991019168908004721
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- Web of Science research areas
- Microbiology