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Antiviral and anti-inflammatory effects of Cannabidiol in HIV/SIV infection
Journal article   Peer reviewed

Antiviral and anti-inflammatory effects of Cannabidiol in HIV/SIV infection

Alysha L. Ellison, José Javier Rosado-Franco, Labib Mamun, Stephen Knerler, Marzieh Daniali, Kiara Mehta, Sierra Williams-McLeod, Jade Alvarez, Savoya S. Joyner, Ryan Vandrey, …
Brain, behavior, and immunity, v 137, 106843
Oct 2026
PMID: 42235652
Featured in Collection :   Drexel's Newest Publications
url
https://doi.org/10.1016/j.bbi.2026.106843View
Published, Version of Record (VoR) Restricted CC BY-NC V4.0

Abstract

Cannabidiol Endocannabinoid HIV SIV Inflammation
•Cannabidiol (CBD) suppresses simian immunodeficiency virus (SIV) replication and during acute infection of rhesus macaques.•CBD maintain cellular immunity in vivo during acute SIV infection.•CBD modulates endocannabinoid reporters during acute SIV infection.•CBD suppresses human immunodeficiency virus (HIC) replication of primary human cells.•CBD selectively suppresses inflammatory cytokines in primary human cells. Persistent reservoirs and chronic immune activation are hallmarks of HIV, despite the effectiveness of antiretroviral therapy (ART) in suppressing viral replication. Here, we use rhesus macaques and primary and induced pluripotent stem cell (iPSC)-derived human immune cells to evaluate the virologic and immunologic consequences of cannabidiol (CBD) exposure during HIV/SIV infection. We show that CBD, in the absence of ART, suppresses viral replication and establishment of the viral reservoir to levels comparable with first-line therapies during acute SIV infection of rhesus macaques. This antiviral effect of CBD extended to in vitro HIV infection of human macrophages, T cells, and microglia. Immunologically, we observe CBD slowed CD4 + T cell decline and polarization, decreased CD14 + CD16 + monocyte expansion, and reduced interferon-inducible cytokine release in rhesus macaques. We identify comparable effects on cytokine production with in vitro CBD treatment of human macrophages, T cells, and microglia. Importantly, we find CBD inhibits cytokines only when an immune response is elicited by HIV, suggesting it is not broadly immunosuppressive. Finally, we determine CBD regulates endocannabinoid receptors, modulators, and transporters and inhibits NF-κb and STAT1 activation when mediating its antiviral and anti-inflammatory effects. These findings show beneficial effects of CBD in laboratory models of untreated HIV, thus placebo-controlled clinical trials to evaluate the safety and effectiveness of adjunctive CBD use with ART is warranted.

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