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Journal article
Apigenin Modulates Dendritic Cell Activities and Curbs Inflammation Via RelB Inhibition in the Context of Neuroinflammatory Diseases
Journal of neuroimmune pharmacology, v 16(2), pp 403-424
01 Jun 2021
PMID: 32607691
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Neuroinflammation leads to tissue injury causing many of the clinical symptoms of Multiple Sclerosis, an autoimmune disorder of the central nervous system (CNS). While T cells, specifically T(h)1 and T(h)17 cells, are the ultimate effectors of this disease, dendritic cells (DCs) mediate T cell polarization, activation, etc. In our previous study, Apigenin, a natural flavonoid, has been shown to reduce EAE disease severity through amelioration of demyelination in the CNS as well as the sequestering of DCs and other myeloid cells in the periphery. Here, we show that Apigenin exerts its effects possibly through shifting DC modulated T cell responses from T(h)1 and T(h)17 type towards T(reg)directed responses evident through the decrease in T-bet, IFN-gamma (T(h)1),IL-17 (T(h)17) and increase in IL-10, TGF-beta and FoxP3 (T-reg) expression in cells from both normal human donors and EAE mice. RelB, an NF-kappa beta pathway protein is central to DC maturation, its antigen presentation capabilities and DC-mediated T cell activation. Apigenin reduced mRNA and protein levels of RelB and also reduced its nuclear translocation. Additionally, siRNA-mediated silencing of RelB further potentiated the RelB-mediated effects of Apigenin thus confirming its role in Apigenin directed regulation of DC biology. These results provide key information about the molecular events controlled by Apigenin in its regulation of DC activity marking its potential as a therapy for neuroinflammatory disease.
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Details
- Title
- Apigenin Modulates Dendritic Cell Activities and Curbs Inflammation Via RelB Inhibition in the Context of Neuroinflammatory Diseases
- Creators
- Rashida Ginwala - Drexel UniversityRaina Bhavsar - Drexel UniversityPatrick Moore - Drexel UniversityMariana Bernui - Drexel UniversityNarendra Sing - Univ South Carolina, Dept Pathol Microbiol & Immunol, Columbia, SC 29208 USAFrank Bearoff - Drexel UniversityMitzi Nagarkatti - Univ South Carolina, Dept Pathol Microbiol & Immunol, Columbia, SC 29208 USAZafar K. Khan - Drexel UniversityPooja Jain - Drexel University
- Publication Details
- Journal of neuroimmune pharmacology, v 16(2), pp 403-424
- Publisher
- Springer Nature
- Number of pages
- 22
- Grant note
- R01 NS097147 / NIH/NINDS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) RG 4471A6/2 / National MS Society; National Multiple Sclerosis Society
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000544613500001
- Scopus ID
- 2-s2.0-85087319781
- Other Identifier
- 991019169666304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences
- Pharmacology & Pharmacy