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Apigenin, a Natural Flavonoid, Attenuates EAE Severity Through the Modulation of Dendritic Cell and Other Immune Cell Functions
Journal article   Open access   Peer reviewed

Apigenin, a Natural Flavonoid, Attenuates EAE Severity Through the Modulation of Dendritic Cell and Other Immune Cell Functions

Rashida Ginwala, Emily McTish, Chander Raman, Narendra Singh, Mitzi Nagarkatti, Prakash Nagarkatti, Divya Sagar, Pooja Jain and Zafar K Khan
Journal of neuroimmune pharmacology, v 11(1)
Mar 2016
PMID: 26040501
url
https://doi.org/10.1007/s11481-015-9617-xView
Published, Version of Record (VoR) Open

Abstract

Immunohistochemistry Encephalomyelitis, Autoimmune, Experimental - pathology Anti-Inflammatory Agents - pharmacology Dendritic Cells - immunology Mice, Inbred C57BL Encephalomyelitis, Autoimmune, Experimental - immunology Spinal Cord - immunology Animals Spinal Cord - pathology Brain - pathology Dendritic Cells - drug effects Female Mice Brain - immunology Apigenin - pharmacology
Apigenin, a natural flavonoid, found in several plants, fruits, vegetables, herbs, and spices, is known to have anti-oxidant and anti-inflammatory properties that are evident in the use of these substances for centuries as medicinal approaches to treat asthma, insomnia, Parkinson's disease, neuralgia, and shingles. However, there is a considerable dearth of information regarding its effect on immune cells, especially dendritic cells (DC) that maintain the critical balance between an immunogenic and tolerogenic immune response, in an immunospecialized location like the central nervous system (CNS). In this paper we looked at the anti-inflammatory properties of Apigenin in restoration of immune function and the resultant decrease in neuroinflammation. In vivo, a significant reduction in severity of experimental autoimmune encephalomyelitis (EAE) progression and relapse was observed in C57BL/6 (progressive) and SJL/J (relapse-remitting) mouse models of multiple sclerosis upon treatment with Apigenin. Apigenin treated EAE mice show decreased expression of α4 integrin and CLEC12A on splenic DCs and an increased retention of immune cells in the periphery compared to untreated EAE mice. This correlated consequently with immunohistochemistry findings of decreased immune cell infiltration and reduced demyelination in the CNS. These results indicate a protective role of Apigenin against the neurodegenerative effects resulting from the entry of DC stimulated pathogenic T cells into the CNS thus implicating a potential therapy for neuroinflammatory disease.

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Collaboration types
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Web of Science research areas
Neurosciences
Pharmacology & Pharmacy
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