Journal article
Apoptosis in cerebellar granule cells is blocked by high KCl, forskolin, and IGF-1 through distinct mechanisms of action: the involvement of intracellular calcium and RNA synthesis
The Journal of neuroscience, v 15(2), pp 1172-1179
01 Feb 1995
PMID: 7532699
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cerebellar granule cells deprived of depolarizing concentration of extracellular potassium, [K+]o, undergo apoptosis. We here report that this apoptotic process is associated with an immediate and permanent decrease in the levels of free intracellular calcium, [Ca2+]i. Although forskolin and IGF-1 are both able to prevent apoptosis, only forskolin is able to counteract the instantaneous decrease of [Ca2+]i. However, the early effect of forskolin on [Ca2+]i is lost after longer incubation in low [K+]o. The calcium antagonist nifedipine is able to inhibit the survival effect of high [K+]o, while not affecting forskolin and IGF-1 promoted survival, as assessed by viability and genomic DNA analysis. Accordingly, the L-type calcium channels agonist Bay K8644 significantly enhanced the survival of low KCl treated neurons. To temporally characterize the signal transduction events and the essential transcriptional step in cerebellar granule cells apoptosis, we determined the time course of the rescue capacity of high [K+]o, forskolin, IGF-1, and actinomycin D. Addition of high KCl, forskolin, or IGF-1 6 hr after the initial KCl deprivation saves 50% of cells. Remarkably, 50% of neurons loss the potential to be rescued by actinomycin D after only 1 hr in low [K+]o. Finally, we show that the survival promoting activities of high [K+]o, forskolin, and IGF-1 do not require RNA synthesis. We conclude that [Ca2+]i is involved in the survival promoting activity exerted by high [K+]o but not in those of forskolin and IGF-1, and that all three agents, although rescuing neurons from apoptosis through distinct mechanisms of action, do not necessitate RNA transcription.
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Details
- Title
- Apoptosis in cerebellar granule cells is blocked by high KCl, forskolin, and IGF-1 through distinct mechanisms of action: the involvement of intracellular calcium and RNA synthesis
- Creators
- C Galli - Institute of Neurobiology-CNR, Rome, ItalyO Meucci - Institute of Neurobiology-CNR, Rome, ItalyA Scorziello - Institute of Neurobiology-CNR, Rome, ItalyT M Werge - Institute of Neurobiology-CNR, Rome, ItalyP Calissano - Institute of Neurobiology-CNR, Rome, ItalyG Schettini - Institute of Neurobiology-CNR, Rome, Italy
- Publication Details
- The Journal of neuroscience, v 15(2), pp 1172-1179
- Publisher
- Society for Neuroscience
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1995QH37100021
- Scopus ID
- 2-s2.0-0028924891
- Other Identifier
- 991014878372804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences