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Apoptotic and anti-apoptotic mechanisms following spinal cord injury
Journal article   Open access   Peer reviewed

Apoptotic and anti-apoptotic mechanisms following spinal cord injury

Robert Keane, Susan Kraydieh, George Lotocki and John Bethea
Journal of neuropathology and experimental neurology, v 60(5), pp 422-429
01 May 2001
DOI: https://doi.org/10.1093/jnen/60.5.422
PMID: 11379817
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://academic.oup.com/jnen/article-pdf/60/5/422/9552292/60-5-422.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1093/jnen/60.5.422View
Published, Version of Record (VoR) Open

Abstract

A number of studies have provided evidence that cell death from moderate traumatic spinal cord injury (SCI) is regulated, in part, by apoptosis that involves the caspase family of cysteine proteases. However, little or no information is available about anti-apoptotic mechanisms mediated by the inhibitors of apoptosis (IAP) family of proteins that inhibit cell death pathways. In the present study, we examined caspase and IAP expression in spinal cords of rats subjected to moderate traumatic injury. Within 6 h after injury, caspase-8 and-9 (2 initiators of apoptosis) were predominantly present in gray matter neurons within the lesion epicenter. By 3 days following spinal cord injury (SCI), caspase-8 and-9 immunoreactivity was localized to gray and white matter cells, and by 7 days following SCI, both upstream caspases were expressed in cells within white matter or within foamy macrophages in gray matter. Caspase-3, an effector caspase, was evident in a few fragmented cells in gray matter at 24 h following injury and then localized to white matter in later stages. Thus, distinct patterns of caspase expression can be found in the spinal cord following injury. XIAP, cIAP-1, and cIAP-2, members of the IAP family, were constitutively expressed in the cord. Immunoblots of spinal cord extracts revealed that the processed forms of caspases-8 and-9 and cleavage of PARP are present as early as 6 h following trauma. The expression of caspases corresponded with the detection of cleavage of XIAP into 2 fragments following injury. cIAP-1 and cIAP-2 expression remained constant during early periods following SCI but demonstrated alterations by 7 days following SCI. Our data are consistent with the idea that XIAP may have a protective role within the spinal cord, and that alteration in cleavage of XIAP may regulate cell death following SCI.

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130 citations in Web of Science
142 citations in Scopus

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UN Sustainable Development Goals (SDGs)

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#3 Good Health and Well-Being

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Collaboration types
Domestic collaboration
Web of Science research areas
Clinical Neurology
Neurosciences
Pathology
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