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Journal article
Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research
Frontiers in oncology, v 10, pp 878-878
03 Jun 2020
PMID: 32582546
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The existing
in vitro
models for antitumor drug screening have great limitations. Many compounds that inhibit 2D cultured cells do not exhibit the same pharmacological effects
in vivo
, thereby wasting human and material resources as well as time during drug development. Therefore, developing new models is critical. The 3D bioprinting technology has greater advantages in constructing human tissue compared with sandwich culture and organoid construction. Here, we used 3D bioprinting technology to construct a 3D model with HepG2 cells (3DP-HepG2). The biological activities of the model were evaluated by immunofluorescence, real-time quantitative PCR, and transcriptome sequencing. Compared with the traditional 2D cultured tumor cells (2D-HepG2), 3DP-HepG2 showed significantly improved expression of tumor-related genes, including ALB, AFP, CD133, IL-8, EpCAM, CD24, and β-TGF genes. Transcriptome sequencing analysis revealed large differences in gene expression between 3DP-HepG2 and 2D-HepG2, especially genes related to hepatocyte function and tumor. We also compared the effects of antitumor drugs in 3DP-HepG2 and 2D-HepG2, and found that the large differences in drug resistance genes between the models may cause differences in the drugs' pharmacodynamics.
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Details
- Title
- Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research
- Creators
- Lejia Sun - Peking Union Medical College HospitalHuayu Yang - Peking Union Medical College HospitalYanan Wang - State Key Laboratory of Medical Molecular Biology, Department of Physiology, Institute of Basic Medical Sciences, CAMS and School of Basic Medicine, PUMC, Beijing, China.Xinyu Zhang - State Key Laboratory of Medical Molecular Biology, Department of Physiology, Institute of Basic Medical Sciences, CAMS and School of Basic Medicine, PUMC, Beijing, China.Bao Jin - Peking Union Medical College HospitalFeihu Xie - Peking Union Medical College HospitalYukai Jin - Peking Union Medical College HospitalYuan Pang - Tsinghua UniversityHaitao Zhao - Peking Union Medical College HospitalXin Lu - Peking Union Medical College HospitalXinting Sang - Peking Union Medical College HospitalHongbing Zhang - State Key Laboratory of Medical Molecular Biology, Department of Physiology, Institute of Basic Medical Sciences, CAMS and School of Basic Medicine, PUMC, Beijing, China.Feng Lin - Tsinghua UniversityWei Sun - Tsinghua UniversityPengyu Huang - Center for Excellence in Molecular Cell ScienceYilei Mao - Peking Union Medical College Hospital
- Publication Details
- Frontiers in oncology, v 10, pp 878-878
- Publisher
- Frontiers Media S.A
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000543201400001
- Scopus ID
- 2-s2.0-85086588641
- Other Identifier
- 991019167590304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Oncology