Journal article
Arabidopsis thaliana expresses two functional isoforms of Arvp, a protein involved in the regulation of cellular lipid homeostasis
Biochimica et biophysica acta. Molecular and cell biology of lipids, v 1761(7), pp 725-735
Jul 2006
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Arv1p is involved in the regulation of cellular lipid homeostasis in the yeast
Saccharomyces cerevisiae. Here, we report the characterization of the two
Arabidopsis thaliana ARV genes and the encoded proteins, AtArv1p and AtArv2p. The functional identity of AtArv1p and AtArv2p was demonstrated by complementation of the thermosensitive phenotype of the
arv1Δ yeast mutant strain YJN1756. Both
A. thaliana proteins contain the bipartite Arv1 homology domain (AHD), which consists of an NH
2-terminal cysteine-rich subdomain with a putative zinc-binding motif followed by a C-terminal subdomain of 33 amino acids. Removal of the cysteine-rich subdomain has no effect on Arvp activity, whereas the presence of the C-terminal subdomain of the AHD is critical for Arvp function. Localization experiments of AtArv1p and AtArv2p tagged with green fluorescent protein (GFP) and expressed in onion epidermal cells demonstrated that both proteins are exclusively targeted to the endoplasmic reticulum. Analysis of β-glucuronidase (GUS) activity in transgenic
A. thaliana plants carrying chimeric
ARV1::
GUS and
ARV2::
GUS genes showed that
ARV gene promoters direct largely overlapping patterns of expression that are restricted to tissues in which cells are actively dividing or expanding. The results of this study support the notion that plants, yeast and mammals share common molecular mechanisms regulating intracellular lipid homeostasis.
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Details
- Title
- Arabidopsis thaliana expresses two functional isoforms of Arvp, a protein involved in the regulation of cellular lipid homeostasis
- Creators
- Oriol Forés - Universitat de BarcelonaMontserrat Arró - Universitat de BarcelonaAlbert Pahissa - Universitat de BarcelonaSergi Ferrero - Universitat de BarcelonaMelody Germann - Drexel UniversityJoseph Stukey - Hope CollegeVirginia McDonough - Hope CollegeJoseph T. Nickels - Drexel UniversityNarciso Campos - Universitat de BarcelonaAlbert Ferrer - Universitat de Barcelona
- Publication Details
- Biochimica et biophysica acta. Molecular and cell biology of lipids, v 1761(7), pp 725-735
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000240166200009
- Scopus ID
- 2-s2.0-33746313123
- Other Identifier
- 991021229885204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- Cell Biology