Journal article
Array lead zirconate titanate∕glass piezoelectric microcantilevers for real-time detection of Bacillus anthracis with 10 spores∕ml sensitivity and 1∕1000 selectivity in bacterial mixtures
Review of scientific instruments, v 80(12), pp 125104-125104-7
Dec 2009
PMID: 20059167
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
An array of three identical piezoelectric microcantilever sensors (PEMSs) consisting of a lead zirconate titanate layer bonded to a glass layer was fabricated and examined for simultaneous,
in situ
, real-time, all-electrical detection of
Bacillus anthracis
(BA) spores in an aqueous suspension using the first longitudinal extension mode of resonance. With anti-BA antibody immobilized on the sensor surfaces all three PEMS exhibited identical BA detection resonance frequency shifts at all tested concentrations, 10–10
7
spores∕ml with a standard deviation of less than 10%. The detection concentration limit of 10 spores∕ml was about two orders of magnitude lower than would be permitted by flexural peaks. In blinded-sample testing, the array PEMS detected BA in three samples containing BA: (1) 3.3×10
3
spores∕ml, (2) a mixture of 3.3×10
3
spores∕ml and 3.3×10
5
S. aureus
(SA) and
P. aeruginosa
(PA) per ml, and (3) a mixture of 3.3×10
3
spores∕ml with 3.3×10
6
SA+PA∕ml. There was no response to a sample containing only 3.3×10
6
SA+PA∕ml. These results illustrate the sensitivity, specificity, reusability, and reliability of array PEMS for
in situ
, real-time detection of BA spores.
Metrics
Details
- Title
- Array lead zirconate titanate∕glass piezoelectric microcantilevers for real-time detection of Bacillus anthracis with 10 spores∕ml sensitivity and 1∕1000 selectivity in bacterial mixtures
- Creators
- John-Paul McGovern - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USAWei-Heng Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USARichard F Rest - Department of Microbiology and Immunology, Drexel University, Philadelphia, Pennsylvania 19104, USAMitali Purohit - Department of Microbiology and Immunology, Drexel University, Philadelphia, Pennsylvania 19104, USAMark Mattiucci - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, USAKambiz Pourrezaei - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, USABanu Onaral - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, USAWan Y Shih - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, USA
- Publication Details
- Review of scientific instruments, v 80(12), pp 125104-125104-7
- Publisher
- American Institute of Physics
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; [Retired Faculty]; Materials Science and Engineering
- Web of Science ID
- WOS:000273217300045
- Scopus ID
- 2-s2.0-73849089015
- Other Identifier
- 991014877787104721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Instruments & Instrumentation
- Physics, Applied