Journal article
Array lead zirconate titanate∕glass piezoelectric microcantilevers for real-time detection of Bacillus anthracis with 10 spores∕ml sensitivity and 1∕1000 selectivity in bacterial mixtures
Review of scientific instruments, Vol.80(12), pp.125104-125104-7
Dec 2009
PMCID: PMC2802521
PMID: 20059167
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
An array of three identical piezoelectric microcantilever sensors (PEMSs) consisting of a lead zirconate titanate layer bonded to a glass layer was fabricated and examined for simultaneous,
in situ
, real-time, all-electrical detection of
Bacillus anthracis
(BA) spores in an aqueous suspension using the first longitudinal extension mode of resonance. With anti-BA antibody immobilized on the sensor surfaces all three PEMS exhibited identical BA detection resonance frequency shifts at all tested concentrations, 10–10
7
spores∕ml with a standard deviation of less than 10%. The detection concentration limit of 10 spores∕ml was about two orders of magnitude lower than would be permitted by flexural peaks. In blinded-sample testing, the array PEMS detected BA in three samples containing BA: (1) 3.3×10
3
spores∕ml, (2) a mixture of 3.3×10
3
spores∕ml and 3.3×10
5
S. aureus
(SA) and
P. aeruginosa
(PA) per ml, and (3) a mixture of 3.3×10
3
spores∕ml with 3.3×10
6
SA+PA∕ml. There was no response to a sample containing only 3.3×10
6
SA+PA∕ml. These results illustrate the sensitivity, specificity, reusability, and reliability of array PEMS for
in situ
, real-time detection of BA spores.
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Details
- Title
- Array lead zirconate titanate∕glass piezoelectric microcantilevers for real-time detection of Bacillus anthracis with 10 spores∕ml sensitivity and 1∕1000 selectivity in bacterial mixtures
- Creators
- John-Paul McGovern - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USAWei-Heng Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USARichard F Rest - Department of Microbiology and Immunology, Drexel University, Philadelphia, Pennsylvania 19104, USAMitali Purohit - Department of Microbiology and Immunology, Drexel University, Philadelphia, Pennsylvania 19104, USAMark Mattiucci - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, USAKambiz Pourrezaei - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, USABanu Onaral - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, USAWan Y Shih - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104, USA
- Publication Details
- Review of scientific instruments, Vol.80(12), pp.125104-125104-7
- Publisher
- American Institute of Physics
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering; School of Biomedical Engineering, Science, and Health Systems; [Retired Faculty]
- Identifiers
- 991014877787104721
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- Instruments & Instrumentation
- Physics, Applied