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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Artefactual formation of pyruvate from in-source conversion of lactate
Rapid communications in mass spectrometry, v 32(14), pp 1163-1168
30 Jul 2018
PMID: 29740888
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
RationaleLactate and pyruvate are high-abundance products of glucose metabolism. Analysis of both molecules as part of metabolomics studies in cellular metabolism and physiology have been aided by advances in liquid chromatography/mass spectrometry (LC/MS).
MethodsWe used ion-pairing chromatography and negative ion mode electrospray ionization (ESI) on a QExactive HF to perform stable isotope assisted metabolomics profiling of lactate and pyruvate metabolism.
ResultsUsing an LC/MS method for polar metabolite analysis we discovered an artefactual formation of pyruvate from in-source fragmentation of lactate. Surprisingly, this in-source fragmentation has not been previously described, thus we report this identification to warn other investigators. This artefact was detected by baseline chromatographic resolution of lactate and pyruvate by LC with confirmation of this artefact by stable isotope labeling of lactate and pyruvate.
ConclusionsThese findings have immediate implications for metabolomics studies by LC/MS and direct infusion MS, especially in negative ion mode, whereby users should resolve lactate from pyruvate or robustly quantify the potential formation of pyruvate from higher abundance lactate in their assays.
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Details
- Title
- Artefactual formation of pyruvate from in-source conversion of lactate
- Creators
- Sophie Trefely - Drexel UniversityClementina Mesaros - Center for Excellence in Environmental Toxicology, Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Philadelphia, PA 19104, USAPeining Xu - Drexel UniversityMary T. Doan - Drexel UniversityHelen Jiang - Drexel UniversityOya Altinok - Drexel UniversityZulfiya Orynbayeva - Drexel UniversityNathaniel W. Snyder - Drexel University
- Publication Details
- Rapid communications in mass spectrometry, v 32(14), pp 1163-1168
- Publisher
- Wiley
- Number of pages
- 6
- Grant note
- K22ES026235 / National Institute of Environmental Health Sciences; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) R03HD092630 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) R03HD092630 / Eunice Kennedy Shriver National Institute of Child Health and Human Development; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; A.J. Drexel Autism Institute; Surgery
- Web of Science ID
- WOS:000439869900007
- Scopus ID
- 2-s2.0-85061059699
- Other Identifier
- 991019167410404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Spectroscopy