Files and links (1)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Arv1 lipid transporter function is conserved between pathogenic and nonpathogenic fungi
Fungal genetics and biology, v 49(2), pp 101-113
01 Feb 2012
PMID: 22142782
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The lipid transporter Arv1 regulates sterol trafficking, and glycosylphosphatidylinositol and sphingolipid biosyntheses in Saccharomyces cerevisiae. ScArv1 contains an Arv1 homology domain (AHD) that is conserved at the amino acid level in the pathogenic fungal species, Candida albicans and Candida glabrata. Here we show S. cerevisiae cells lacking Arv1 are highly susceptible to antifungal drugs. In the presence of drug, Scarv1 cells are unable to induce ERG gene expression, have an altered pleiotrophic drug response, and are defective in multi-drug resistance efflux pump expression. All phenotypes are remediated by ectopic expression of CaARV1 or CgARV1. The AHDs of these pathogenic fungi are required for specific drug tolerance, demonstrating conservation of function. In order to understand how Arv1 regulates antifungal susceptibility, we examined sterol trafficking. CaARV1/CgARV1 expression suppressed the sterol trafficking defect of Scarv1 cells. Finally, we show that C. albicans arv1/arv1 cells are avirulent using a BALB/c disseminated mouse model. We suggest that overall cell survival in response to antifungal treatment requires the lipid transporter function of Arv1. (C) 2011 Elsevier Inc. All rights reserved.
Metrics
Details
- Title
- Arv1 lipid transporter function is conserved between pathogenic and nonpathogenic fungi
- Creators
- Christina Gallo-Ebert - AnsysPaula C. McCourt - AnsysMelissa Donigan - AnsysMichelle L. Villasmil - AnsysWeiWei Chen - AnsysDevanshi Pandya - AnsysJudith Franco - AnsysDesiree Romano - AnsysSean G. Chadwick - Medical Diagnostic Laboratories (United States)Scott E. Gygax - Medical Diagnostic Laboratories (United States)Joseph T. Nickels - Ansys
- Publication Details
- Fungal genetics and biology, v 49(2), pp 101-113
- Publisher
- Elsevier
- Number of pages
- 13
- Grant note
- HL67401 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01HL067401 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) Genesis Biotechnology Group
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000300749000002
- Scopus ID
- 2-s2.0-84863081183
- Other Identifier
- 991021229987104721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Genetics & Heredity
- Mycology