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Aspiration of a cervical spinal contusion injury in preparation for delayed peripheral nerve grafting does not impair forelimb behavior or axon regeneration
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Aspiration of a cervical spinal contusion injury in preparation for delayed peripheral nerve grafting does not impair forelimb behavior or axon regeneration

Harra R Sandrow, Jed S Shumsky, Arthi Amin and John D Houle
Experimental neurology, v 210(2), pp 489-500
Apr 2008
DOI: https://doi.org/10.1016/j.expneurol.2007.11.029
PMID: 18295206
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1016/j.expneurol.2007.11.029View
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Abstract

Exploratory Behavior Nerve Regeneration - physiology Ectodysplasins - metabolism Rats Axons - physiology Glial Fibrillary Acidic Protein - metabolism Spinal Cord Compression - pathology Spinal Cord Compression - physiopathology Rats, Sprague-Dawley Behavior, Animal Forelimb - physiopathology Peripheral Nerves - transplantation Animals Neurologic Examination Analysis of Variance Glial Cell Line-Derived Neurotrophic Factor - therapeutic use Time Factors Spinal Cord Compression - therapy Female Suction - methods Locomotion - physiology Disease Models, Animal
A peripheral nerve graft model was used to examine axonal growth after a unilateral cervical (C) contusion injury in adult rats and to determine if manipulation of an injury site prior to transplantation affects spontaneous behavioral recovery. After a short delay (7 d) the epicenter of a C4 contusion was exposed and aspirated without harming the cavity walls followed by apposition with one end of a pre-degenerated tibial nerve to the rostral cavity wall. After a longer delay (28 d) the aspirated cavity was treated with GDNF to promote regeneration by chronically injured neurons. In both groups forelimb and hindlimb locomotor scores decreased significantly 2 d after lesion site manipulation, but by 7 d, the forelimb score was not different from the pre-manipulation score. There was no significant difference in grid walking or grip strength scores for the affected forelimb in either group 7 d after contusion vs. 7 d after manipulation. Over 1500 brain stem and propriospinal neurons grew axons into the graft with either delay. These results demonstrate that a contusion injury site can be manipulated prior to transplantation without causing long-lasting forelimb or hindlimb behavioral deficits and that peripheral nerve grafts support axonal growth after acute or chronic contusion injury.

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