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Assays used to study the DNA replication checkpoint in fission yeast
Journal article   Open access

Assays used to study the DNA replication checkpoint in fission yeast

Eishi Noguchi, Alison B Ansbach, Chiaki Noguchi and Paul Russell
Methods in molecular biology (Clifton, N.J.), v 521, pp 493-507
2009
DOI: https://doi.org/10.1007/978-1-60327-815-7_28
PMID: 19563125
url
https://europepmc.org/articles/pmc3582217View
Accepted (AM)Open Access (License Unspecified),  Open

Abstract

Bacterial Proteins - metabolism Checkpoint Kinase 2 DNA Damage DNA Repair DNA Replication DNA, Fungal - biosynthesis DNA-Binding Proteins - metabolism Electrophoresis, Gel, Pulsed-Field Immunoprecipitation Luminescent Proteins - metabolism Mycology - methods Protein-Serine-Threonine Kinases - metabolism Recombinant Fusion Proteins - metabolism S Phase Schizosaccharomyces - cytology Schizosaccharomyces - metabolism Schizosaccharomyces pombe Proteins - metabolism
The DNA replication checkpoint, also known as the intra-S or S-phase checkpoint, plays a central role in ensuring the accuracy of DNA replication. When replication is impeded by DNA damage or other conditions, this checkpoint delays cell cycle progression and coordinates resumption of replication with DNA repair pathways. One of its critical functions is to stabilize stalled replication forks in a replication-competent state, presumably by maintaining proper assembly of replisome components and preserving DNA structures. Here we describe a series of assays used to study the replication checkpoint. These assays allow us to investigate the specific functions of proteins involved in the replication checkpoint in fission yeast.

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