Journal article
Assembly of Hepatitis B Virus Envelope Proteins onto a Lentivirus Pseudotype That Infects Primary Human Hepatocytes
Journal of virology, v 81(20), pp 10897-10904
01 Oct 2007
PMCID: PMC2045538
PMID: 17670822
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This study demonstrates that the envelope proteins of hepatitis B virus (HBV) could be incorporated into the lipid membrane of lentivirus pseudotype particles. The assembly procedure was initiated by the transfection of 293T cells with three plasmids: (i) a human immunodeficiency virus (HIV) packaging construct, (ii) a transfer plasmid expressing a reporter gene, and (iii) a plasmid expressing large (L), middle (M), and small (S) HBV envelope proteins. After 2 days, hepatitis B surface antigen and the antigenic forms of L, M, and S were detected at the cell surface by flow cytometry. Also, virus particles that were able to infect cultured primary human hepatocytes (PHH) were released. Under optimal conditions, 50% of PHH could be infected. In addition, the susceptibility of PHH and the resistance of other cell types to the pseudotype particles were similar to those observed for HBV and hepatitis delta virus (HDV), which shares the same L, M, and S. Furthermore, the infection of PHH by the pseudotype was sensitive to known inhibitors of HBV and HDV entry. These findings of specific and efficient infection of hepatocytes could be applicable to liver-specific gene therapy and may help clarify the attachment and entry mechanism used by HBV and HDV.
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Details
- Title
- Assembly of Hepatitis B Virus Envelope Proteins onto a Lentivirus Pseudotype That Infects Primary Human Hepatocytes
- Creators
- Ning Chai - Fox Chase Cancer CenterHo Eun Chang - Fox Chase Cancer CenterEmmanuelle Nicolas - Fox Chase Cancer CenterSeverin Gudima - Fox Chase Cancer CenterJinhong Chang - Fox Chase Cancer CenterJohn Taylor - Fox Chase Cancer Center
- Publication Details
- Journal of virology, v 81(20), pp 10897-10904
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000250019400007
- Scopus ID
- 2-s2.0-35148812988
- Other Identifier
- 991020547320304721
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- Web of Science research areas
- Virology