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Journal article
Assembly of hepatitis delta virus: particle characterization, including the ability to infect primary human hepatocytes
Journal of virology, v 81(7), pp 3608-3617
01 Apr 2007
PMID: 17229685
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Efficient assembly of hepatitis delta virus (HDV) was achieved by cotransfection of Huh7 cells with two plasmids: one to provide expression of the large, middle, and small envelope proteins of hepatitis B virus (HBV), the natural helper of HDV, and another to initiate replication of the HDV RNA genome. HDV released into the media was assayed for HDV RNA and HBV envelope proteins and characterized by rate-zonal sedimentation, immunoaffinity purification, electron microscopy, and the ability to infect primary human hepatocytes. Among the novel findings were that (i) immunostaining for delta antigen 6 days after infection with 300 genome equivalents (GE) per cell showed only 1% of cells as infected, but this was increased to 16% when 5% polyethylene glycol was present during infection; (ii) uninfected cells did not differ from infected cells in terms of albumin accumulation or the presence of E-cadherin at cell junctions; and (iii) sensitive quantitative real-time PCR assays detected HDV replication even when the multiplicity of infection was 0.2 GE/cell. In the future, this HDV assembly and infection system can be further developed to better understand the mechanisms shared by HBV and HDV for attachment and entry into host cells.
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Details
- Title
- Assembly of hepatitis delta virus: particle characterization, including the ability to infect primary human hepatocytes
- Creators
- Severin Gudima - Fox Chase Cancer CenterYiping He - Fox Chase Cancer CenterAnja Meier - Fox Chase Cancer CenterJinhong Chang - Fox Chase Cancer CenterRongji Chen - Fox Chase Cancer CenterMichal Jarnik - Fox Chase Cancer CenterEmmanuelle Nicolas - Fox Chase Cancer CenterVolker Bruss - University of GöttingenJohn Taylor - Fox Chase Cancer Center
- Publication Details
- Journal of virology, v 81(7), pp 3608-3617
- Publisher
- American Society for Microbiology (ASM)
- Grant note
- P30 CA006927 / NCI NIH HHS AI 058269 / NIAID NIH HHS CA 06927 / NCI NIH HHS U01 AI058269 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000244988100056
- Scopus ID
- 2-s2.0-33947408707
- Other Identifier
- 991020547316404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Virology