Journal article
Assessment of ALK gene fusions in lung cancer using the differential expression and exon integrity methods
Oncology letters, v 11(3), pp 1651-1656
01 Mar 2016
PMID: 26998057
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Anaplastic lymphoma kinase (ALK) gene fusion is a driving mutation underlying the development of non-small cell lung cancer (NSCLC). Accurate detection of ALK fusion is critical for the use of ALK inhibitors in the treatment of NSCLC. Commonly utilized methods for ALK detection include fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC). However, these methods are time-consuming and costly. In the present study, a method for assessing ALK gene fusion based on the differential expression levels of the ALK kinase and non-kinase domains was developed and evaluated, with the aim of providing a convenient and reliable method for the detection of ALK fusion. In addition, another method was established to determine the integrity of exons 19-20 and 20-21 of ALK, two genomic loci that are typically broken in ALK fusions. These novel methods were applied to detect ALK fusion in 100 NSCLC patients, and were compared with IHC and FISH methods. The novel methods developed in the present study successfully detected ALK fusions in 10 samples. The concordances between the novel methods and IHC and FISH were 100%. Furthermore, the differential expression method was able to detect ALK fusions in cell-free urine samples, which was advantageous over FISH and IHC. The novel methods developed in the present study are cost-effective and easy to perform, and may provide simple and convenient techniques for the clinical assessment of ALK fusions, facilitating the use of targeted therapy for NSCLC.
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Details
- Title
- Assessment of ALK gene fusions in lung cancer using the differential expression and exon integrity methods
- Creators
- Qing Huang - Ningbo UniversityQiuhua Deng - First Affiliated Hospital of Guangzhou Medical UniversityLei Jiang - Ningbo UniversityRong Fang - Ningbo UniversityYinghua Qiu - Unigene Biosci Co Ltd, Ningbo 315211, Zhejiang, Peoples R ChinaPing Wang - Ningbo UniversityJeff X. Zhou - Ningbo UniversityHaihong Yang - College Station Medical Center
- Publication Details
- Oncology letters, v 11(3), pp 1651-1656
- Publisher
- Spandidos Publ Ltd
- Number of pages
- 6
- Grant note
- 2013C33165 / Science and Technology Plan of Zhejiang Province of China 81302000 / National Natural Science Fund for Distinguished Young Scholars; National Natural Science Foundation of China (NSFC); National Science Fund for Distinguished Young Scholars
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000371634000006
- Scopus ID
- 2-s2.0-84958068518
- Other Identifier
- 991021463546904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Oncology