Journal article
Association of IL-6 with PM2.5 Components: Importance of Characterizing Filter-Based PM2.5 Following Extraction
Water, air, and soil pollution, v 228(1)
28 Dec 2016
PMID: 28989204
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Filter-based toxicology studies are conducted to establish the biological plausibility of the well-established health impacts associated with fine particulate matter (PM
2.5
) exposure. Ambient PM
2.5
collected on filters is extracted into solution for toxicology applications, but frequently, characterization is nonexistent or only performed on filter-based PM
2.5
, without consideration of compositional differences that occur during the extraction processes. To date, the impact of making associations to measured components in ambient instead of extracted PM
2.5
has not been investigated. Filter-based PM
2.5
was collected at locations (
n
= 5) and detailed characterization of both ambient and extracted PM
2.5
was performed. Alveolar macrophages (AMJ2-C11) were exposed (3, 24, and 48 h) to PM
2.5
and the pro-inflammatory cytokine interleukin (IL)-6 was measured. IL-6 release differed significantly between PM
2.5
collected from different locations; surprisingly, IL-6 release was highest following treatment with PM
2.5
from the lowest ambient concentration location. IL-6 was negatively correlated with the sum of ambient metals analyzed, as well as with concentrations of specific constituents which have been previously associated with respiratory health effects. However, positive correlations of IL-6 with extracted concentrations indicated that the negative associations between IL-6 and ambient concentrations do not accurately represent the relationship between inflammation and PM
2.5
exposure. Additionally, seven organic compounds had significant associations with IL-6 release when considering ambient concentrations, but they were not detected in the extracted solution. Basing inflammatory associations on ambient concentrations that are not necessarily representative of in vitro exposures creates misleading results; this study highlights the importance of characterizing extraction solutions to conduct accurate health impact research.
Metrics
Details
- Title
- Association of IL-6 with PM2.5 Components: Importance of Characterizing Filter-Based PM2.5 Following Extraction
- Creators
- Courtney Roper - University of PittsburghLauren G. Chubb - University of PittsburghLeah Cambal - University of PittsburghBrett Tunno - University of PittsburghJane E. Clougherty - University of PittsburghCheryl Fattman - University of PittsburghSteven E. Mischler - University of Pittsburgh
- Publication Details
- Water, air, and soil pollution, v 228(1)
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Dana and David Dornsife School of Public Health; Drexel University; Environmental and Occupational Health
- Web of Science ID
- WOS:000390681700039
- Scopus ID
- 2-s2.0-85007504754
- Other Identifier
- 991020100086704721
UN Sustainable Development Goals (SDGs)
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Source: SDGs in the Output
InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Environmental Sciences
- Meteorology & Atmospheric Sciences
- Water Resources