Files and links (1)
Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
Association of Purα with RNAs Homologous to 7 SL Determines Its Binding Ability to the Myelin Basic Protein Promoter DNA Sequence
The Journal of biological chemistry, v 273(35), pp 22241-22247
28 Aug 1998
PMID: 9712838
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cell type and developmental stage expression of the myelin basic protein (MBP) gene in mouse brain is regulated at the transcriptional level. Earlier studies from our laboratory have led to the identification of a DNA binding protein from mouse brain, named Purα, which interacts with the MB1 regulatory motif of the MBP and stimulates its transcription in glial cells. In this report, we demonstrate that a cellular RNA, with significant homology to 7 SL RNA is associated with Purα. Results from band shift competition studies indicate that Purα-associated RNA (PU-RNA), inhibits the interaction of immunopurified Purα with the MB1 DNA sequence. Results from Northern blot studies indicated that PU-RNA is expressed during various stages of brain development. Of interest, this RNA was found in association with Purα that was produced in the mouse brain at the early stage of brain development. Results from Northwestern analysis using a PU-RNA probe identified the regions within Purα that are important for Purα/PU-RNA association. Production of Purα at the early stage of brain development and its association with PU-RNA at this stage, when Purα exhibits poor binding ability to the MB1 DNA sequence, suggests that PU-RNA may function as a co-factor that negatively regulates Purα interaction with the MBP promoter sequence.
Metrics
Details
- Title
- Association of Purα with RNAs Homologous to 7 SL Determines Its Binding Ability to the Myelin Basic Protein Promoter DNA Sequence
- Creators
- Anna Tretiakova - Allegheny University of the Health SciencesGary L. Gallia - Allegheny University of the Health SciencesNatalia Shcherbik - Allegheny University of the Health SciencesBradford Jameson - Allegheny University of the Health SciencesEdward M. Johnson - Allegheny University of the Health SciencesShohreh Amini - Allegheny University of the Health SciencesKamel Khalili - Allegheny University of the Health Sciences
- Publication Details
- The Journal of biological chemistry, v 273(35), pp 22241-22247
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000075616600014
- Scopus ID
- 2-s2.0-0032575373
- Other Identifier
- 991019169580304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology