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Atmospheric Nonthermal Plasma-Treated PBS Inactivates Escherichia coli by Oxidative DNA Damage
Journal article   Open access   Peer reviewed

Atmospheric Nonthermal Plasma-Treated PBS Inactivates Escherichia coli by Oxidative DNA Damage

Adam D Yost and Suresh G Joshi
PloS one, v 10(10), pp e0139903-e0139903
2015
DOI: https://doi.org/10.1371/journal.pone.0139903
PMID: 26461113
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1371/journal.pone.0139903View
Published, Version of Record (VoR)CC BY V4.0,  Open

Abstract

Antioxidants - pharmacology Atmosphere - chemistry Buffers Catalase - genetics Cell Membrane - drug effects Cell Membrane - metabolism Cytoprotection - drug effects DNA Damage Escherichia coli - drug effects Escherichia coli - genetics Gene Expression Regulation, Bacterial - drug effects Genes, Bacterial Microbial Viability - drug effects Mutation Oxidation-Reduction - drug effects Oxidative Stress - drug effects Oxidative Stress - genetics Plasma Gases - pharmacology Reactive Oxygen Species - metabolism Stress, Physiological - drug effects Superoxide Dismutase - genetics
We recently reported that phosphate-buffered saline (PBS) treated with nonthermal dielectric-barrier discharge plasma (plasma) acquires strong antimicrobial properties, but the mechanisms underlying bacterial inactivation were not known. The goal of this study is to understand the cellular responses of Escherichia coli and to investigate the properties of plasma-activated PBS. The plasma-activated PBS induces severe oxidative stress in E. coli cells and reactive-oxygen species scavengers, α-tocopherol and catalase, protect E. coli from cell death. Here we show that the response of E. coli to plasma-activated PBS is regulated by OxyR and SoxyRS regulons, and mediated predominantly through the expression of katG that deactivates plasma-generated oxidants. During compensation of E. coli in the absence of both katG and katE, sodA and sodB are significantly overexpressed in samples exposed to plasma-treated PBS. Microarray analysis found that up-regulation of genes involved in DNA repair, and E. coli expressing recA::lux fusion was extremely sensitive to the SOS response upon exposure to plasma-treated PBS. The cellular changes include rapid loss of E. coli membrane potential and membrane integrity, lipid peroxidation, accumulation of 8-hydroxy-deoxyguinosine (8OHdG), and severe oxidative DNA damage; reveal ultimate DNA disintegration, and cell death. Together, these data suggest that plasma-treated PBS contains hydrogen peroxide and superoxide like reactive species or/and their products which lead to oxidative changes to cell components, and are eventually responsible for cell death.

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55 citations in Web of Science
65 citations in Scopus

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UN Sustainable Development Goals (SDGs)

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#3 Good Health and Well-Being

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Web of Science research areas
Microbiology
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