Journal article
Attenuated protein kinase C activity and translocation in Alzheimer's Disease brain
Neurobiology of aging, v 15(3), pp 293-298
1994
PMID: 7936052
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Protein kinase C (PKC) activity and its redistribution were determined in postmortem Alzheimer's disease (AD) and age-matched control brains. Cytosolic and membrane-associated PKC activities were lower in frontal and temporal cortices and hippocampi of AD brains. Increased concentrations of phosphatidyl-
l-serine, Ca
2+ or phorbol 12-myristate, 13-acetate only weakly increased enzyme activity in AD tissues. Redistribution of cytosolic PKC to the membranous fraction was elicited in control brain slices by 162 nM PMA in the presence of K
+ (65 mM). This redistribution of the enzyme was markedly reduced in AD brain slices. In contrast, the immunoreactivity of the α- and γ-PKC isozymes were elevated in cortical tissue from AD subjects. No changes were noted in β-PKC immunoreactivity. These results suggest that the reduced PKC activity and the attenuated translocation of the enzyme in AD brain tissue may be attributed to down regulation of PKC or to alteration in PKC protein. The increase in PKC immunoreactivity may be a reflection of an altered susceptibility to proteolysis or a compensatory response secondary to the loss in enzyme activity.
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Details
- Title
- Attenuated protein kinase C activity and translocation in Alzheimer's Disease brain
- Creators
- Hoau-Yan Wang - Drexel UniversityMichael R. Pisano - Drexel UniversityEitan Friedman - Drexel University
- Publication Details
- Neurobiology of aging, v 15(3), pp 293-298
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1994NQ03300001
- Scopus ID
- 2-s2.0-0028339544
- Other Identifier
- 991019184078904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Geriatrics & Gerontology
- Neurosciences