Journal article
Attenuation of Verapamil‐induced Myocardial Toxicity in an Ex‐vivo Rat Model Using a Verapamil‐specific Ovine Immunoglobin
Academic emergency medicine, v 8(10), pp 950-955
Oct 2001
PMID: 11581079
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Objective: To determine whether an ovine verapamil‐specific immunoglobin G (V‐IgG) attenuates verapamil toxicity in an ex‐vivo rat left ventricular papillary muscle model. Methods: The authors dissected left ventricular papillary muscle strips from male Sprague‐Dawley rats (350‐410 g) and suspended them in an oxygen‐perfused Tyrode buffer bath at 37.5°C. Muscle strips equilibrated for 90 minutes under electrical stimulation of 1 Hz. Resting and developed tension (mg) were monitored continuously. A concentration—response trial was performed with verapamil concentrations ranging from 31 to 1,020 nM; 510 nM produced consistent reduction in developed tension. A trial of V‐IgG was then conducted by administering the following treatments to papillary muscle strips in a randomized manner: V‐IgG + 510 nM verapamil, nonspecific ovine IgG (N‐IgG) + 510 nM verapamil (protein control), and 510 nM verapamil alone. Immunoglobin G was administered in equimolar concentrations to verapamil. Attenuation was expressed as inhibition of the verapamil‐induced reduction of developed tension. Results: The V‐IgG comparative trial indicated the V‐IgG + verapamil treatment had a mean reduction in developed tension of 14.1% (SD ± 12.2) compared with 36.2% (SD ± 14.9) for N‐IgG + verapamil and 34.9% (SD ± 8.1) for verapamil alone (p < 0.05). There was no difference between the two control groups. Conclusion: Verapamil‐specific IgG attenuated verapamil‐induced reduction of developed tension in an ex‐vivo rat model.
Metrics
Details
- Title
- Attenuation of Verapamil‐induced Myocardial Toxicity in an Ex‐vivo Rat Model Using a Verapamil‐specific Ovine Immunoglobin
- Creators
- Robert E. Hill - University of Colorado HealthKennon Heard - University of Colorado HealthGregory M. Bogdan - University of Colorado HealthCharles B. Cairns - University of Colorado HealthRichard C. Dart - Denver Health Medical Center
- Publication Details
- Academic emergency medicine, v 8(10), pp 950-955
- Publisher
- Blackwell Publishing Ltd
- Number of pages
- 6
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine
- Web of Science ID
- WOS:000171444600003
- Scopus ID
- 2-s2.0-0034800218
- Other Identifier
- 991021463568104721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Emergency Medicine